Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
Authors
Naveas, Nelson
Pulido, Ruth
Torres-Costa, Vicente
Agullo-Rueda, Fernando
Santibanez, Mauricio
Malano, Francisco
Recio-Sanchez, Gonzalo
Garrido-Miranda, Karla A.
Manso-Silvan, Miguel
Hernandez-Montelongo, Jacobo
Pulido, Ruth
Torres-Costa, Vicente
Agullo-Rueda, Fernando
Santibanez, Mauricio
Malano, Francisco
Recio-Sanchez, Gonzalo
Garrido-Miranda, Karla A.
Manso-Silvan, Miguel
Hernandez-Montelongo, Jacobo
Profesor GuĆa
Authors
Date
Datos de publicaciĆ³n:
10.3390/ijms241310595
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,Vol.24,2023
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,Vol.24,2023
Tipo de recurso
Article
Keywords
Materia geogrƔfica
Collections
Abstract
The design and engineering of antibacterial materials are key for preventing bacterial adherence and proliferation in biomedical and household instruments. Silver nanoparticles (AgNPs) and chitosan (CHI) are broad-spectrum antibacterial materials with different properties whose combined application is currently under optimization. This study proposes the formation of antibacterial films with AgNPs embedded in carboxymethylcellulose/chitosan multilayers by the layer-by-layer (LbL) method. The films were deposited onto nanoporous silicon (nPSi), an ideal platform for bioengineering applications due to its biocompatibility, biodegradability, and bioresorbability. We focused on two alternative multilayer deposition processes: cyclic dip coating (CDC) and cyclic spin coating (CSC). The physicochemical properties of the films were the subject of microscopic, microstructural, and surface-interface analyses. The antibacterial activity of each film was investigated against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria strains as model microorganisms. According to the findings, the CDC technique produced multilayer films with higher antibacterial activity for both bacteria compared to the CSC method. Bacteria adhesion inhibition was observed from only three cycles. The developed AgNPs-multilayer composite film offers advantageous antibacterial properties for biomedical applications.