Effect of sperm treatment with lysolecithin on in vitro outcomes of equine intracytoplasmic sperm injection
Date
Authors
SILVA JIMENEZ, MAURICIO
Águila, Luis
Cabrera, Paulina
Árias, María Elena
Silva, Mauricio Erciario
Felmer, Ricardo N.
Águila, Luis
Cabrera, Paulina
Árias, María Elena
Silva, Mauricio Erciario
Felmer, Ricardo N.
Authors
Date
Datos de publicación:
10.1016/j.jevs.2024.105095
Keywords
Embryo Production - Heterologous Fertilization - Horse Ivf - Sperm Treatment - Dna - Lysophosphatidylcholine - Lysophosphatidylcholines - Dna - Lysophosphatidylcholine - Acrosome - Animal Cell - Article - Blood Level - Cell Degeneration - Cell Membrane - Concentration (parameter) - Dna Fragmentation - Embryo - Female - Flow Cytometry - Gamete - Horse - In Vitro Study - Insemination - Intracytoplasmic Sperm Injection - Male - Nonhuman - Oocyte Activation - Oocyte Maturation - Seminal Plasma - Slaughterhouse - Sperm Preparation - Sperm Quality - Spermatozoon - Staining - Zygote - Animal - Drug Effect - Oocyte - Procedures - Veterinary Medicine - Animals - Female - Horses - Lysophosphatidylcholines - Male - Oocytes - Sperm Injections, Intracytoplasmic - Spermatozoa
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Abstract
Intracytoplasmic sperm injection (ICSI) in horses is currently employed for clinical and commercial uses, but the protocol could be optimized to improve its efficiency. We have hypothesized that destabilization of plasma and acrosomal membranes prior to injection would positively impact the developmental potential of equine zygotes generated by ICSI. This study evaluated effects of the sperm treatment with lysolecithin on plasma and acrosomal membranes and on oocyte activation ability, initially following heterologous ICSI on bovine oocytes and subsequently employing equine oocytes. The effects of the lysolecithin -treatment on the efficiency of conventional and piezo-assisted equine ICSI were evaluated. To do this, the equine sperm were treated with different concentrations of lysolecithin and the sperm plasma membrane, acrosome and DNA integrity were evaluated by flow cytometry. The results showed that a lysolecithin concentration of 0.08 % destabilized the membranes of all sperm and affected DNA integrity within the range described for the species (8 30 %). In addition, the heterologous ICSI assay showed that lysolecithin treatment was detrimental to the sperm's ability to activate the oocyte, therefore, chemical oocyte activation was used after equine ICSI after injection with lysolecithin -treated sperm. This group showed similar developmental rate to the control group with and without exogenous activation. In conclusion, lysolecithin pre-treatment is not necessary when using ICSI to produce equine embryos in vitro. The results from the current study provide additional insight regarding the factors impacting ICSI in horses. © 2024 Elsevier B.V., All rights reserved.
Description
Keywords
Embryo Production , Heterologous Fertilization , Horse Ivf , Sperm Treatment , Dna , Lysophosphatidylcholine , Lysophosphatidylcholines , Dna , Lysophosphatidylcholine , Acrosome , Animal Cell , Article , Blood Level , Cell Degeneration , Cell Membrane , Concentration (parameter) , Dna Fragmentation , Embryo , Female , Flow Cytometry , Gamete , Horse , In Vitro Study , Insemination , Intracytoplasmic Sperm Injection , Male , Nonhuman , Oocyte Activation , Oocyte Maturation , Seminal Plasma , Slaughterhouse , Sperm Preparation , Sperm Quality , Spermatozoon , Staining , Zygote , Animal , Drug Effect , Oocyte , Procedures , Veterinary Medicine , Animals , Female , Horses , Lysophosphatidylcholines , Male , Oocytes , Sperm Injections, Intracytoplasmic , Spermatozoa
Citation
10.1016/j.jevs.2024.105095