Effect of sperm treatment with lysolecithin on in vitro outcomes of equine intracytoplasmic sperm injection

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datacite.alternateIdentifier.citationJournal of Equine Veterinary Science, 138, 2024
datacite.alternateIdentifier.doi10.1016/j.jevs.2024.105095
datacite.alternateIdentifier.issn0737-0806
datacite.creatorÁguila, Luis
datacite.creatorCabrera, Paulina
datacite.creatorÁrias, María Elena
datacite.creatorSilva, Mauricio Erciario
datacite.creatorFelmer, Ricardo N.
datacite.date2024
datacite.rightsRegistro bibliográfico
datacite.subjectEmbryo Production
datacite.subjectHeterologous Fertilization
datacite.subjectHorse Ivf
datacite.subjectSperm Treatment
datacite.subjectDna
datacite.subjectLysophosphatidylcholine
datacite.subjectLysophosphatidylcholines
datacite.subjectDna
datacite.subjectLysophosphatidylcholine
datacite.subjectAcrosome
datacite.subjectAnimal Cell
datacite.subjectArticle
datacite.subjectBlood Level
datacite.subjectCell Degeneration
datacite.subjectCell Membrane
datacite.subjectConcentration (parameter)
datacite.subjectDna Fragmentation
datacite.subjectEmbryo
datacite.subjectFemale
datacite.subjectFlow Cytometry
datacite.subjectGamete
datacite.subjectHorse
datacite.subjectIn Vitro Study
datacite.subjectInsemination
datacite.subjectIntracytoplasmic Sperm Injection
datacite.subjectMale
datacite.subjectNonhuman
datacite.subjectOocyte Activation
datacite.subjectOocyte Maturation
datacite.subjectSeminal Plasma
datacite.subjectSlaughterhouse
datacite.subjectSperm Preparation
datacite.subjectSperm Quality
datacite.subjectSpermatozoon
datacite.subjectStaining
datacite.subjectZygote
datacite.subjectAnimal
datacite.subjectDrug Effect
datacite.subjectOocyte
datacite.subjectProcedures
datacite.subjectVeterinary Medicine
datacite.subjectAnimals
datacite.subjectFemale
datacite.subjectHorses
datacite.subjectLysophosphatidylcholines
datacite.subjectMale
datacite.subjectOocytes
datacite.subjectSperm Injections, Intracytoplasmic
datacite.subjectSpermatozoa
datacite.titleEffect of sperm treatment with lysolecithin on in vitro outcomes of equine intracytoplasmic sperm injection
dc.contributor.authorSILVA JIMENEZ, MAURICIO
dc.description.abstractIntracytoplasmic sperm injection (ICSI) in horses is currently employed for clinical and commercial uses, but the protocol could be optimized to improve its efficiency. We have hypothesized that destabilization of plasma and acrosomal membranes prior to injection would positively impact the developmental potential of equine zygotes generated by ICSI. This study evaluated effects of the sperm treatment with lysolecithin on plasma and acrosomal membranes and on oocyte activation ability, initially following heterologous ICSI on bovine oocytes and subsequently employing equine oocytes. The effects of the lysolecithin -treatment on the efficiency of conventional and piezo-assisted equine ICSI were evaluated. To do this, the equine sperm were treated with different concentrations of lysolecithin and the sperm plasma membrane, acrosome and DNA integrity were evaluated by flow cytometry. The results showed that a lysolecithin concentration of 0.08 % destabilized the membranes of all sperm and affected DNA integrity within the range described for the species (8 30 %). In addition, the heterologous ICSI assay showed that lysolecithin treatment was detrimental to the sperm's ability to activate the oocyte, therefore, chemical oocyte activation was used after equine ICSI after injection with lysolecithin -treated sperm. This group showed similar developmental rate to the control group with and without exogenous activation. In conclusion, lysolecithin pre-treatment is not necessary when using ICSI to produce equine embryos in vitro. The results from the current study provide additional insight regarding the factors impacting ICSI in horses. © 2024 Elsevier B.V., All rights reserved.
dc.description.ia_keywordsperm, icsi, lysolecithin, equine, treatment, injection, plasma
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5983
dc.language.isoen
dc.publisherElsevier BV
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceJournal of Equine Veterinary Science
dc.subject.ia_oecd1nCiencias Médicas y de la Salud
dc.subject.ia_oecd2nMedicina Clínica
dc.subject.ia_oecd3nMedicina Clínica
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2024
oaire.citationTitleJournal of Equine Veterinary Science
oaire.citationVolume138
oaire.fundingReferenceANID FONDECYT 1160467
oaire.fundingReferenceANID FONDECYT INICIACIÓN 11230091
oaire.fundingReferenceUFRO PROGRAMA FORMACIÓN INVESTIGADORES POSTDOCTORALES PDT21-0001
oaire.licenseConditionCopyright © Elsevier Inc, 2024
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication5a5fa19b-bce1-4387-8139-279b35112838
relation.isAuthorOfPublication.latestForDiscovery5a5fa19b-bce1-4387-8139-279b35112838
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed/MEDLINE
uct.indizacionCAB Abstracts
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