Isolation and molecular characterization of MYB60 in Solanum lycopersicum
Date
Authors
INOSTROZA BLANCHETEAU, CLAUDIO ANDRES
Rodríguez-Hoces de la Guardia, Amparo
Ugalde, María Beatriz
Lobos-Diaz, Valeria
Romero-Romero, Jesús Lucina
Meyer-Regueiro, Carlos
Inostroza-Blancheteau, Claudio
Reyes-Díaz, Marjorie M.
Aquea, Felipe
Arce-Johnson, Patricio
Rodríguez-Hoces de la Guardia, Amparo
Ugalde, María Beatriz
Lobos-Diaz, Valeria
Romero-Romero, Jesús Lucina
Meyer-Regueiro, Carlos
Inostroza-Blancheteau, Claudio
Reyes-Díaz, Marjorie M.
Aquea, Felipe
Arce-Johnson, Patricio
Authors
Date
Datos de publicación:
10.1007/s11033-021-06168-5
Keywords
Abiotic Stress - Drought - Myb60 - Stomatal Aperture - Tomato - Abscisic Acid - Abscisic Acid - Arabidopsis Proteins - Myb60 Protein, Arabidopsis - Transcription Factors - Arabidopsis - Article - Cellular Distribution - Controlled Study - Gene Control - Gene Expression - Gene Isolation - Gene Location - Gene Repression - Gene Sequence - Gene Targeting - In Vitro Study - Myb60 Gene - Nonhuman - Nucleotide Sequence - Phenotype - Plant Gene - Plant Leaf - Plant Stoma - Promoter Region - Real Time Polymerase Chain Reaction - Regulatory Sequence - Tomato - Adaptation - Drought - Gene Expression Regulation - Genetics - Growth, Development And Aging - Metabolism - Transgenic Plant - Abscisic Acid - Arabidopsis Protein - Myb60 Protein, Arabidopsis - Transcription Factor - Abscisic Acid - Adaptation, Physiological - Arabidopsis Proteins - Droughts - Gene Expression Regulation, Plant - Lycopersicon Esculentum - Plants, Genetically Modified - Regulatory Sequences, Nucleic Acid - Transcription Factors
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Abstract
Stomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops. © 2021 Elsevier B.V., All rights reserved.
Description
Keywords
Abiotic Stress , Drought , Myb60 , Stomatal Aperture , Tomato , Abscisic Acid , Abscisic Acid , Arabidopsis Proteins , Myb60 Protein, Arabidopsis , Transcription Factors , Arabidopsis , Article , Cellular Distribution , Controlled Study , Gene Control , Gene Expression , Gene Isolation , Gene Location , Gene Repression , Gene Sequence , Gene Targeting , In Vitro Study , Myb60 Gene , Nonhuman , Nucleotide Sequence , Phenotype , Plant Gene , Plant Leaf , Plant Stoma , Promoter Region , Real Time Polymerase Chain Reaction , Regulatory Sequence , Tomato , Adaptation , Drought , Gene Expression Regulation , Genetics , Growth, Development And Aging , Metabolism , Transgenic Plant , Abscisic Acid , Arabidopsis Protein , Myb60 Protein, Arabidopsis , Transcription Factor , Abscisic Acid , Adaptation, Physiological , Arabidopsis Proteins , Droughts , Gene Expression Regulation, Plant , Lycopersicon Esculentum , Plants, Genetically Modified , Regulatory Sequences, Nucleic Acid , Transcription Factors
Citation
10.1007/s11033-021-06168-5