Isolation and molecular characterization of MYB60 in Solanum lycopersicum

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datacite.alternateIdentifier.citationMolecular Biology Reports, 48 (2), 1587-1579, 2021
datacite.alternateIdentifier.doi10.1007/s11033-021-06168-5
datacite.alternateIdentifier.issn1573-4978
datacite.creatorRodríguez-Hoces de la Guardia, Amparo
datacite.creatorUgalde, María Beatriz
datacite.creatorLobos-Diaz, Valeria
datacite.creatorRomero-Romero, Jesús Lucina
datacite.creatorMeyer-Regueiro, Carlos
datacite.creatorInostroza-Blancheteau, Claudio
datacite.creatorReyes-Díaz, Marjorie M.
datacite.creatorAquea, Felipe
datacite.creatorArce-Johnson, Patricio
datacite.date2021
datacite.rightsRegistro bibliográfico
datacite.subjectAbiotic Stress
datacite.subjectDrought
datacite.subjectMyb60
datacite.subjectStomatal Aperture
datacite.subjectTomato
datacite.subjectAbscisic Acid
datacite.subjectAbscisic Acid
datacite.subjectArabidopsis Proteins
datacite.subjectMyb60 Protein, Arabidopsis
datacite.subjectTranscription Factors
datacite.subjectArabidopsis
datacite.subjectArticle
datacite.subjectCellular Distribution
datacite.subjectControlled Study
datacite.subjectGene Control
datacite.subjectGene Expression
datacite.subjectGene Isolation
datacite.subjectGene Location
datacite.subjectGene Repression
datacite.subjectGene Sequence
datacite.subjectGene Targeting
datacite.subjectIn Vitro Study
datacite.subjectMyb60 Gene
datacite.subjectNonhuman
datacite.subjectNucleotide Sequence
datacite.subjectPhenotype
datacite.subjectPlant Gene
datacite.subjectPlant Leaf
datacite.subjectPlant Stoma
datacite.subjectPromoter Region
datacite.subjectReal Time Polymerase Chain Reaction
datacite.subjectRegulatory Sequence
datacite.subjectTomato
datacite.subjectAdaptation
datacite.subjectDrought
datacite.subjectGene Expression Regulation
datacite.subjectGenetics
datacite.subjectGrowth, Development And Aging
datacite.subjectMetabolism
datacite.subjectTransgenic Plant
datacite.subjectAbscisic Acid
datacite.subjectArabidopsis Protein
datacite.subjectMyb60 Protein, Arabidopsis
datacite.subjectTranscription Factor
datacite.subjectAbscisic Acid
datacite.subjectAdaptation, Physiological
datacite.subjectArabidopsis Proteins
datacite.subjectDroughts
datacite.subjectGene Expression Regulation, Plant
datacite.subjectLycopersicon Esculentum
datacite.subjectPlants, Genetically Modified
datacite.subjectRegulatory Sequences, Nucleic Acid
datacite.subjectTranscription Factors
datacite.titleIsolation and molecular characterization of MYB60 in Solanum lycopersicum
dc.contributor.authorINOSTROZA BLANCHETEAU, CLAUDIO ANDRES
dc.description.abstractStomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops. © 2021 Elsevier B.V., All rights reserved.
dc.description.ia_keywordslmyb, expression, stomatal, plants, regulation, transcription, arabidopsis
dc.identifier.issn0301-4851
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4020
dc.language.isoen
dc.publisherSpringer Nature
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceMolecular Biology Reports
dc.subject.ia_odsODS 8: Trabajo decente y crecimiento económico
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nBiología General
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2021
oaire.citationEndPage1587
oaire.citationIssue2
oaire.citationStartPage1579
oaire.citationTitleMolecular Biology Reports
oaire.citationVolume48
oaire.fundingReferenceCONICYT Beca Doctorado Nacional
oaire.fundingReferenceUNAB Fellowship
oaire.fundingReferenceANID FONDECYT 1150220, 11160355, 1171286 (Regular)
oaire.fundingReferenceANID BASAL FB0002
oaire.licenseConditionCopyright © Springer Nature, 2021
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication25287ee1-cae8-401d-b42c-ee3f8fc05d2a
relation.isAuthorOfPublication.latestForDiscovery25287ee1-cae8-401d-b42c-ee3f8fc05d2a
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Agropecuarias y Acuícolas
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionDOAJ
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