Administration of Beta-Nerve Growth Factor during the Preovulatory Stage Improves Endocrine and Luteal Function in Dairy Heifers
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Authors
SILVA JIMENEZ, MAURICIO
Gajardo, Gonzalo
Paiva, Luis
Ulloa-Leal, Cesar
Valderrama, Ximena P.
López, Gerardo
Carrasco, Albert
Hidalgo, Alejandra Isabel
Silva, Mauricio Erciario
Palma, Patricio I.
Ratto, M. Hector
Gajardo, Gonzalo
Paiva, Luis
Ulloa-Leal, Cesar
Valderrama, Ximena P.
López, Gerardo
Carrasco, Albert
Hidalgo, Alejandra Isabel
Silva, Mauricio Erciario
Palma, Patricio I.
Ratto, M. Hector
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Date
Datos de publicación:
10.3390/ani13061004
Keywords
Artificial Insemination - Bovine - Neurotrophin - Pregnancy - Reproductive Biotechnology - Estradiol - Estradiol Benzoate - Lipoprotein Lipase - Luteinizing Hormone - Progesterone - Affinityscript - Imagej - Nanodrop 1000 - Quantstudio 3 - Thermocycler - Trizol - Estradiol - Estradiol Benzoate - Gaba Transporter 1 - Lipoprotein Lipase - Luteinizing Hormone - Membrane Protein - Nerve Growth Factor Beta Subunit - Progesterone - Progesterone Receptor - Solute Carrier Family 6 Member 14 - Unclassified Drug - Animal Experiment - Animal Model - Animal Tissue - Article - Artificial Insemination - B Scan - Biotechnology - Blood Sampling - Corpus Luteum - Endocrine Function - Enzyme Linked Immunosorbent Assay - Fast Protein Liquid Chromatography - Female - Gene Expression - Heifer - Holstein-friesian Cattle - Hormone Determination - Isolation And Purification - Luteal Phase - Mrna Expression Level - Nonhuman - Ovulation - Pregnancy Rate - Preovulatory Stage - Radioimmunoassay - Real Time Reverse Transcription Polymerase Chain Reaction - Rna Extraction - Solid Phase Radioimmunoassay - Transrectal Ultrasonography - Uterus Biopsy
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Abstract
The neurotrophin beta-nerve growth factor (NGF), which is present in the semen of different mammals, elicits potent ovulatory and luteotrophic actions in llamas following systemic administration. Here, we determine if purified NGF given intramuscularly (IM) during the preovulatory stage affects the corpus luteum (CL), hormone production, endometrial gene expression, and pregnancy rate of dairy heifers. Holstein-Friesian heifers were estrus-synchronized using estradiol benzoate (EB) plus an intravaginal progesterone (P4) device (DIB). After eight days, the device was removed and cloprostenol was given IM; the next day (day 9), heifers received EB IM plus one of the following: (i) 1 mg of NGF (NGF D9 group), (ii) 1 mg of NGF 32 h after EB (NGF D10 group), or (iii) phosphate buffer saline (control group). To measure pregnancy rates, heifers were treated similarly, then artificially inseminated with sexed semen 48 52 h after DIB removal, then an ultrasound was conducted 30 days after insemination. The females given NGF along with EB (NGF D9) showed significantly higher luteinizing hormone (LH) concentrations, larger CL vascular areas, and higher plasma P4 concentrations than the NGF D10 and control animals. Downregulation of the P4 receptor (PGR), and upregulation of both lipoprotein lipase (LPL) and Solute Carrier Family 6 member 14 (SLC6A14) endometrial genes, were detected in NGF D9 heifers. Furthermore, these heifers had a 10% higher pregnancy rate than the control group. We conclude that the higher P4 output, in response to the early NGF administration, led to the enhanced gene expression of transcripts related to uterine receptivity that may result in enhanced pregnancy rates. © 2023 Elsevier B.V., All rights reserved.
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Keywords
Artificial Insemination , Bovine , Neurotrophin , Pregnancy , Reproductive Biotechnology , Estradiol , Estradiol Benzoate , Lipoprotein Lipase , Luteinizing Hormone , Progesterone , Affinityscript , Imagej , Nanodrop 1000 , Quantstudio 3 , Thermocycler , Trizol , Estradiol , Estradiol Benzoate , Gaba Transporter 1 , Lipoprotein Lipase , Luteinizing Hormone , Membrane Protein , Nerve Growth Factor Beta Subunit , Progesterone , Progesterone Receptor , Solute Carrier Family 6 Member 14 , Unclassified Drug , Animal Experiment , Animal Model , Animal Tissue , Article , Artificial Insemination , B Scan , Biotechnology , Blood Sampling , Corpus Luteum , Endocrine Function , Enzyme Linked Immunosorbent Assay , Fast Protein Liquid Chromatography , Female , Gene Expression , Heifer , Holstein-friesian Cattle , Hormone Determination , Isolation And Purification , Luteal Phase , Mrna Expression Level , Nonhuman , Ovulation , Pregnancy Rate , Preovulatory Stage , Radioimmunoassay , Real Time Reverse Transcription Polymerase Chain Reaction , Rna Extraction , Solid Phase Radioimmunoassay , Transrectal Ultrasonography , Uterus Biopsy
Citation
10.3390/ani13061004