Administration of Beta-Nerve Growth Factor during the Preovulatory Stage Improves Endocrine and Luteal Function in Dairy Heifers

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datacite.alternateIdentifier.citationAnimals, 13 (6), 2023
datacite.alternateIdentifier.doi10.3390/ani13061004
datacite.alternateIdentifier.issn2076-2615
datacite.creatorGajardo, Gonzalo
datacite.creatorPaiva, Luis
datacite.creatorUlloa-Leal, Cesar
datacite.creatorValderrama, Ximena P.
datacite.creatorLópez, Gerardo
datacite.creatorCarrasco, Albert
datacite.creatorHidalgo, Alejandra Isabel
datacite.creatorSilva, Mauricio Erciario
datacite.creatorPalma, Patricio I.
datacite.creatorRatto, M. Hector
datacite.date2023
datacite.rightsAcceso abierto
datacite.subjectArtificial Insemination
datacite.subjectBovine
datacite.subjectNeurotrophin
datacite.subjectPregnancy
datacite.subjectReproductive Biotechnology
datacite.subjectEstradiol
datacite.subjectEstradiol Benzoate
datacite.subjectLipoprotein Lipase
datacite.subjectLuteinizing Hormone
datacite.subjectProgesterone
datacite.subjectAffinityscript
datacite.subjectImagej
datacite.subjectNanodrop 1000
datacite.subjectQuantstudio 3
datacite.subjectThermocycler
datacite.subjectTrizol
datacite.subjectEstradiol
datacite.subjectEstradiol Benzoate
datacite.subjectGaba Transporter 1
datacite.subjectLipoprotein Lipase
datacite.subjectLuteinizing Hormone
datacite.subjectMembrane Protein
datacite.subjectNerve Growth Factor Beta Subunit
datacite.subjectProgesterone
datacite.subjectProgesterone Receptor
datacite.subjectSolute Carrier Family 6 Member 14
datacite.subjectUnclassified Drug
datacite.subjectAnimal Experiment
datacite.subjectAnimal Model
datacite.subjectAnimal Tissue
datacite.subjectArticle
datacite.subjectArtificial Insemination
datacite.subjectB Scan
datacite.subjectBiotechnology
datacite.subjectBlood Sampling
datacite.subjectCorpus Luteum
datacite.subjectEndocrine Function
datacite.subjectEnzyme Linked Immunosorbent Assay
datacite.subjectFast Protein Liquid Chromatography
datacite.subjectFemale
datacite.subjectGene Expression
datacite.subjectHeifer
datacite.subjectHolstein-friesian Cattle
datacite.subjectHormone Determination
datacite.subjectIsolation And Purification
datacite.subjectLuteal Phase
datacite.subjectMrna Expression Level
datacite.subjectNonhuman
datacite.subjectOvulation
datacite.subjectPregnancy Rate
datacite.subjectPreovulatory Stage
datacite.subjectRadioimmunoassay
datacite.subjectReal Time Reverse Transcription Polymerase Chain Reaction
datacite.subjectRna Extraction
datacite.subjectSolid Phase Radioimmunoassay
datacite.subjectTransrectal Ultrasonography
datacite.subjectUterus Biopsy
datacite.titleAdministration of Beta-Nerve Growth Factor during the Preovulatory Stage Improves Endocrine and Luteal Function in Dairy Heifers
dc.contributor.authorSILVA JIMENEZ, MAURICIO
dc.date.accessioned2025-10-06T14:21:40Z
dc.date.available2025-10-06T14:21:40Z
dc.description.abstractThe neurotrophin beta-nerve growth factor (NGF), which is present in the semen of different mammals, elicits potent ovulatory and luteotrophic actions in llamas following systemic administration. Here, we determine if purified NGF given intramuscularly (IM) during the preovulatory stage affects the corpus luteum (CL), hormone production, endometrial gene expression, and pregnancy rate of dairy heifers. Holstein-Friesian heifers were estrus-synchronized using estradiol benzoate (EB) plus an intravaginal progesterone (P4) device (DIB). After eight days, the device was removed and cloprostenol was given IM; the next day (day 9), heifers received EB IM plus one of the following: (i) 1 mg of NGF (NGF D9 group), (ii) 1 mg of NGF 32 h after EB (NGF D10 group), or (iii) phosphate buffer saline (control group). To measure pregnancy rates, heifers were treated similarly, then artificially inseminated with sexed semen 48 52 h after DIB removal, then an ultrasound was conducted 30 days after insemination. The females given NGF along with EB (NGF D9) showed significantly higher luteinizing hormone (LH) concentrations, larger CL vascular areas, and higher plasma P4 concentrations than the NGF D10 and control animals. Downregulation of the P4 receptor (PGR), and upregulation of both lipoprotein lipase (LPL) and Solute Carrier Family 6 member 14 (SLC6A14) endometrial genes, were detected in NGF D9 heifers. Furthermore, these heifers had a 10% higher pregnancy rate than the control group. We conclude that the higher P4 output, in response to the early NGF administration, led to the enhanced gene expression of transcripts related to uterine receptivity that may result in enhanced pregnancy rates. © 2023 Elsevier B.V., All rights reserved.
dc.description.ia_keywordheifers, pregnancy, after, group, higher, administration, given
dc.formatPDF
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6734
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.sourceAnimals
dc.subject.ia_oecd1nCiencias Sociales
dc.subject.ia_oecd2nEconomía y Negocios
dc.subject.ia_oecd3nNegocios y Gestión
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2023
oaire.citationIssue6
oaire.citationTitleAnimals
oaire.citationVolume13
oaire.fundingReferenceANID FONDECYT 1190980
oaire.licenseConditionObra bajo licencia Creative Commons Atribución 4.0 Internacional
oaire.licenseCondition.urihttps://creativecommons.org/licenses/by/4.0/
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
relation.isAuthorOfPublication5a5fa19b-bce1-4387-8139-279b35112838
relation.isAuthorOfPublication.latestForDiscovery5a5fa19b-bce1-4387-8139-279b35112838
uct.catalogadorjvu
uct.comunidadRecursos Naturalesen_US
uct.departamentoDepartamento de Ciencias Veterinarias y Salud Pública
uct.facultadFacultad de Recursos Naturales
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionPubMed
uct.indizacionDOAJ
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