Controlled Release of Caffeic Acid and Pinocembrin by Use of nPSi-?CD Composites Improves Their Antiangiogenic Activity
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Authors
HERNANDEZ MONTELONGO, JESUS JACOBO
Guzmán-Oyarzo, Dina
Hernández-Montelongo, Jacobo
Rosas, Carlos C.
Leal-Rojas, Pamela A.
Weber, Helga L.
Alvear, Marysol
Salazar Navarrete, Luis A.
Guzmán-Oyarzo, Dina
Hernández-Montelongo, Jacobo
Rosas, Carlos C.
Leal-Rojas, Pamela A.
Weber, Helga L.
Alvear, Marysol
Salazar Navarrete, Luis A.
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Date
Datos de publicación:
10.3390/pharmaceutics14030484
Keywords
Antiangiogenic Activity - Caffeic Acid - Controlled Release - Huvecs - Npsi/?cd Microparticle - Pinocembrin - Polyphenols - Beta Cyclodextrin - Caffeic Acid - Chitosan - Hydrogen Peroxide - Pinocembrine - Polyphenol - Angiogenesis Inhibitor - Antioxidant - Beta Cyclodextrin - Caffeic Acid - Chitosan - Hydrogen Peroxide - Nanoporous Material - Nanoporous Silicon - Pinocembrine - Polyphenol - Unclassified Drug - Abts Radical Scavenging Assay - Angiogenesis - Antiangiogenic Activity - Antioxidant Activity - Article - Chorioallantoic Membrane Assay - Controlled Drug Release - Controlled Study - Cytotoxicity - Gene Targeting - Human - Human Cell - Huvec Cell Line - Incubation Time - Microencapsulation - Mts Assay - Oxidative Stress - Physical Chemistry - Real Time Polymerase Chain Reaction
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Abstract
Although polyphenols have great pharmacological potential, the main disadvantage is that they have low bioavailability at the desired site. Thus, the use of biocompatible systems for drug delivery is a strategy that is currently gaining great interest. The objective of this study is to evaluate the effect of microencapsulation of caffeic acid and pinocembrin on the antioxidant and antiangiogenic activity of both polyphenols, by the use of nPSi-?CD composite microparticles. For this HUVEC, cells were exposed to H2O2 and to treatments with polyphenols in solution and loaded in the composite microparticle. The polyphenols were incorporated into a microparticle using nanoporous silicon, chitosan and a ?-cyclodextrin polymer as the biomaterial. The evaluation of the antiangiogenic effect of the treatments with polyphenols in solution and microencapsulated was carried out through functional tests, and the changes in the expression of target genes associated with the antioxidant pathway and angiogenesis was performed through qPCR. The results obtained show that the caffeic acid and pinocembrin have an antioxidant and antiangiogenic activity, both in solution as microencapsulated. In the caffeic acid, a greater biological effect was observed when it was incorporated into the nPSi-?CD composite microparticle. Our results suggest that the nPSi-?CD composite microparticle could be used as an alternative oral drug administration system. © 2022 Elsevier B.V., All rights reserved.
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Keywords
Antiangiogenic Activity , Caffeic Acid , Controlled Release , Huvecs , Npsi/?cd Microparticle , Pinocembrin , Polyphenols , Beta Cyclodextrin , Caffeic Acid , Chitosan , Hydrogen Peroxide , Pinocembrine , Polyphenol , Angiogenesis Inhibitor , Antioxidant , Beta Cyclodextrin , Caffeic Acid , Chitosan , Hydrogen Peroxide , Nanoporous Material , Nanoporous Silicon , Pinocembrine , Polyphenol , Unclassified Drug , Abts Radical Scavenging Assay , Angiogenesis , Antiangiogenic Activity , Antioxidant Activity , Article , Chorioallantoic Membrane Assay , Controlled Drug Release , Controlled Study , Cytotoxicity , Gene Targeting , Human , Human Cell , Huvec Cell Line , Incubation Time , Microencapsulation , Mts Assay , Oxidative Stress , Physical Chemistry , Real Time Polymerase Chain Reaction
Citation
10.3390/pharmaceutics14030484