Transcriptional analysis of flagellar and putative virulence genes of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii
Date
Authors
Medina, Gustavo A.
Neves, Patrícia Regina
Flores-Martin, Sandra N.
Manosalva, Carolina
Andaur, Marcela R.
Otth, Carola
Lincopan, Nilton E.
Fernández, Heriberto
Neves, Patrícia Regina
Flores-Martin, Sandra N.
Manosalva, Carolina
Andaur, Marcela R.
Otth, Carola
Lincopan, Nilton E.
Fernández, Heriberto
Authors
Date
Datos de publicación:
10.1007/s00203-019-01678-0
Keywords
Acanthamoeba - Arcobacter - Endosymbiosis - Flagellar Genes - Virulence Genes - Flagellin - Bacterial Outer Membrane Proteins - Bacterial Proteins - Flaa Protein, Bacteria - Flab1 Protein, Bacteria - Flagellin - Mota Protein, Bacteria - Virulence Factors - Acanthamoeba Castellanii - Arcobacter Butzleri - Article - Endosymbiosis - Nonhuman - Quantitative Analysis - Real Time Polymerase Chain Reaction - Transcription Initiation - Upregulation - Virulence - Animal - Arcobacter - Flagellum - Genetics - Isolation And Purification - Microbiology - Pathogenicity - Symbiosis - Bacterial Protein - Flaa Protein, Bacteria - Flab1 Protein, Bacteria - Flagellin - Mota Protein, Bacteria - Outer Membrane Protein - Virulence Factor - Animals - Bacterial Outer Membrane Proteins - Bacterial Proteins - Flagella - Flagellin - Symbiosis - Virulence - Virulence Factors
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Abstract
Arcobacter butzleri is an emerging foodborne zoonotic pathogen that has been isolated from environmental water sources. This pathogen establishes in vitro endosymbiotic relationships with Acanthamoeba castellanii, a free-living amoeba found in environmental matrices such as soil and water. The principal aim of this study was to analyse the transcriptional pattern of flagellar (flaA-flaB-flgH-motA) and other putative virulence genes (ciaB-cadF-mviN-pldA) of A. butzleri during its interaction with A. castellanii by quantitative real-time PCR. The transcriptional analysis showed up-regulation of all genes analysed before A. butzleri became established as an endocytobiont of A. castellanii. In contrast, while A. butzleri remains an endocytobiont, a significant and sustained decrease in the transcription of all analysed genes was observed. Our findings suggest that A. butzleri requires a biphasic transcriptional pattern of flagellar and other putative virulence genes to establish an endosymbiotic relationship with A. castellanii. © 2019 Elsevier B.V., All rights reserved.
Description
Keywords
Acanthamoeba , Arcobacter , Endosymbiosis , Flagellar Genes , Virulence Genes , Flagellin , Bacterial Outer Membrane Proteins , Bacterial Proteins , Flaa Protein, Bacteria , Flab1 Protein, Bacteria , Flagellin , Mota Protein, Bacteria , Virulence Factors , Acanthamoeba Castellanii , Arcobacter Butzleri , Article , Endosymbiosis , Nonhuman , Quantitative Analysis , Real Time Polymerase Chain Reaction , Transcription Initiation , Upregulation , Virulence , Animal , Arcobacter , Flagellum , Genetics , Isolation And Purification , Microbiology , Pathogenicity , Symbiosis , Bacterial Protein , Flaa Protein, Bacteria , Flab1 Protein, Bacteria , Flagellin , Mota Protein, Bacteria , Outer Membrane Protein , Virulence Factor , Animals , Bacterial Outer Membrane Proteins , Bacterial Proteins , Flagella , Flagellin , Symbiosis , Virulence , Virulence Factors
Citation
10.1007/s00203-019-01678-0