Transcriptional analysis of flagellar and putative virulence genes of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii

Simple item page
datacite.alternateIdentifier.citationArchives of Microbiology, 201 (8), 1083-1075, 2019
datacite.alternateIdentifier.doi10.1007/s00203-019-01678-0
datacite.alternateIdentifier.issn1432-072X
datacite.creatorMedina, Gustavo A.
datacite.creatorNeves, Patrícia Regina
datacite.creatorFlores-Martin, Sandra N.
datacite.creatorManosalva, Carolina
datacite.creatorAndaur, Marcela R.
datacite.creatorOtth, Carola
datacite.creatorLincopan, Nilton E.
datacite.creatorFernández, Heriberto
datacite.date2019
datacite.rightsRegistro bibliográfico
datacite.subjectAcanthamoeba
datacite.subjectArcobacter
datacite.subjectEndosymbiosis
datacite.subjectFlagellar Genes
datacite.subjectVirulence Genes
datacite.subjectFlagellin
datacite.subjectBacterial Outer Membrane Proteins
datacite.subjectBacterial Proteins
datacite.subjectFlaa Protein, Bacteria
datacite.subjectFlab1 Protein, Bacteria
datacite.subjectFlagellin
datacite.subjectMota Protein, Bacteria
datacite.subjectVirulence Factors
datacite.subjectAcanthamoeba Castellanii
datacite.subjectArcobacter Butzleri
datacite.subjectArticle
datacite.subjectEndosymbiosis
datacite.subjectNonhuman
datacite.subjectQuantitative Analysis
datacite.subjectReal Time Polymerase Chain Reaction
datacite.subjectTranscription Initiation
datacite.subjectUpregulation
datacite.subjectVirulence
datacite.subjectAnimal
datacite.subjectArcobacter
datacite.subjectFlagellum
datacite.subjectGenetics
datacite.subjectIsolation And Purification
datacite.subjectMicrobiology
datacite.subjectPathogenicity
datacite.subjectSymbiosis
datacite.subjectBacterial Protein
datacite.subjectFlaa Protein, Bacteria
datacite.subjectFlab1 Protein, Bacteria
datacite.subjectFlagellin
datacite.subjectMota Protein, Bacteria
datacite.subjectOuter Membrane Protein
datacite.subjectVirulence Factor
datacite.subjectAnimals
datacite.subjectBacterial Outer Membrane Proteins
datacite.subjectBacterial Proteins
datacite.subjectFlagella
datacite.subjectFlagellin
datacite.subjectSymbiosis
datacite.subjectVirulence
datacite.subjectVirulence Factors
datacite.titleTranscriptional analysis of flagellar and putative virulence genes of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii
dc.date.accessioned2025-10-06T14:21:43Z
dc.date.available2025-10-06T14:21:43Z
dc.description.abstractArcobacter butzleri is an emerging foodborne zoonotic pathogen that has been isolated from environmental water sources. This pathogen establishes in vitro endosymbiotic relationships with Acanthamoeba castellanii, a free-living amoeba found in environmental matrices such as soil and water. The principal aim of this study was to analyse the transcriptional pattern of flagellar (flaA-flaB-flgH-motA) and other putative virulence genes (ciaB-cadF-mviN-pldA) of A. butzleri during its interaction with A. castellanii by quantitative real-time PCR. The transcriptional analysis showed up-regulation of all genes analysed before A. butzleri became established as an endocytobiont of A. castellanii. In contrast, while A. butzleri remains an endocytobiont, a significant and sustained decrease in the transcription of all analysed genes was observed. Our findings suggest that A. butzleri requires a biphasic transcriptional pattern of flagellar and other putative virulence genes to establish an endosymbiotic relationship with A. castellanii. © 2019 Elsevier B.V., All rights reserved.
dc.description.ia_keywordbutzleri, castellanii, genes, transcriptional, flagellar, putative, virulence
dc.identifier.issn0302-8933
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/6766
dc.language.isoen
dc.publisherSpringer Nature
dc.relationinstname: ANID
dc.relationreponame: Repositorio Digital RI2.0
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.sourceArchives of Microbiology
dc.subject.ia_odsODS 6: Agua limpia y saneamiento
dc.subject.ia_oecd1nCiencias Naturales
dc.subject.ia_oecd2nCiencias Biológicas
dc.subject.ia_oecd3nEcología
dc.type.driverinfo:eu-repo/semantics/article
dc.type.driverhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaireinfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
oaire.citationEdition2019
oaire.citationEndPage1083
oaire.citationIssue8
oaire.citationStartPage1075
oaire.citationTitleArchives of Microbiology
oaire.citationVolume201
oaire.fundingReferenceANID FONDECYT 1110202 (Regular)
oaire.fundingReferenceCONICYT AT 24121322
oaire.fundingReferenceUniversidad Católica de Temuco FEQUIP2018-PL-06
oaire.licenseConditionCopyright © Springer-Verlag GmbH, 2019
oaire.resourceTypeArtículo
oaire.resourceType.enArticle
uct.catalogadorjvu
uct.comunidadCiencias de la Saluden_US
uct.departamentoDepartamento de Procesos Diagnósticos y Evaluación
uct.facultadFacultad de Ciencias de la Salud
uct.indizacionScience Citation Index Expanded - SCIE
uct.indizacionScopus
uct.indizacionScimago
uct.indizacionPubMed
uct.indizacionEMBASE
uct.indizacionLatindex
Files
Collections
Procesos Diagnósticos y Evaluación