Toward the use of mixed microbial cultures for the biological production of adipic and levulinic acid
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Date
Authors
PINTO IBIETA, FERNANDA EMILIA
Pinto-Ibieta, Fernanda
Cea, Mara
Serrano, Antonio
Felissia, Fernando E.
Area, Maria Cristina
Cabrera, Francisco
Ciudad, Gustavo A.
Pinto-Ibieta, Fernanda
Cea, Mara
Serrano, Antonio
Felissia, Fernando E.
Area, Maria Cristina
Cabrera, Francisco
Ciudad, Gustavo A.
Authors
Date
Datos de publicación:
10.3389/fmicb.2023.1224543
Keywords
Adipic Acid - Feast And Famine - Hemicellulose Hydrolysate - Levulinic Acid - Mixed Microbial Cultures (mmc) - Adipic Acid - Cellobiose - Furfural - Levulinic Acid - Lignocellulose - Xylose - Adipic Acid - Cellobiose - Furfural - Levulinic Acid - Lignocellulose - Polyhydroxybutyric Acid - Xylose - Article - Bacterial Growth - Biosynthesis - Carbon Source - Chemical Oxygen Demand - Gas Chromatography - Hunger - Mixed Cell Culture - Mixed Microbial Culture - Nonhuman - Retention Time - Waste Water Treatment Plant
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Abstract
Biological synthesis of high added-value compounds like adipic acid (AA), levulinic acid (LA), or polyhydroxybutyrate (PHB) using pure culture has been separately reported. However, pure culture requires sterile conditions and the use of specific carbon sources resulting in high operating costs. Different alternatives based on the use of mixed microbial cultures (MMC) have been explored to resolve this problem. MMC have been widely reported for the production of PHB, but scarcely reported for LA production and never for AA synthesis. This work presents a novel strategy for the co-production of AA LA, and PHB using MMC. The strategy consists in selecting an MMC producer of AA, LA and PHB from an inoculum obtained from a wastewater treatment plant, which is then subjected to the feast and famine culture strategy in a sequential batch reactor, coupled with a batch reactor step to enhance the accumulation of AA and LA. The results showed that the MMC could produce a 16 ± 2, 23 ± 1 and 5 ± %1 (g compound/g volatile solids) of AA, LA and PHB, respectively, using a non-fermented residual biomass rich in pentose, namely synthetic hemicellulose hydrolysate (SHH) as the carbon source. These results contribute to generating future research to better understand and optimise the biosynthesis of these compounds by MMC. © 2023 Elsevier B.V., All rights reserved.
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Keywords
Adipic Acid , Feast And Famine , Hemicellulose Hydrolysate , Levulinic Acid , Mixed Microbial Cultures (mmc) , Adipic Acid , Cellobiose , Furfural , Levulinic Acid , Lignocellulose , Xylose , Adipic Acid , Cellobiose , Furfural , Levulinic Acid , Lignocellulose , Polyhydroxybutyric Acid , Xylose , Article , Bacterial Growth , Biosynthesis , Carbon Source , Chemical Oxygen Demand , Gas Chromatography , Hunger , Mixed Cell Culture , Mixed Microbial Culture , Nonhuman , Retention Time , Waste Water Treatment Plant
Citation
10.3389/fmicb.2023.1224543