Cryostorage of white cachama (Piaractus orinoquensis) sperm: Effects on cellular, biochemical and ultrastructural parameters

Simple item page
datacite.alternateIdentifier.citationAQUACULTURE REPORTS,Vol.29,,2023
datacite.alternateIdentifier.doi10.1016/j.aqrep.2023.101477
datacite.creatorMedina Robles, Victor Mauricio
datacite.creatorSandoval Vargas, Leydy Yasmin
datacite.creatorSuarez Martinez, Roger Oswaldo
datacite.creatorGomez Ramirez, Edwin
datacite.creatorGuaje Ramirez, Diana Nataly
datacite.creatorCruz Casallas, Pablo Emilio
datacite.date2023
datacite.subject.englishCryostorage time
datacite.subject.englishCryostorage damage
datacite.subject.englishSperm cryopreservation
datacite.subject.englishSperm ultrastructure
datacite.subject.englishPost-thaw sperm quality
datacite.titleCryostorage of white cachama (Piaractus orinoquensis) sperm: Effects on cellular, biochemical and ultrastructural parameters
dc.date.accessioned2023-06-08T15:48:18Z
dc.date.available2023-06-08T15:48:18Z
dc.description.abstractTo date, little attention has been paid to identifying the effects of long-term cryopreservation on sperm quality for Piaractus orinoquensis. The object of this study was therefore to evaluate the effect of long-term cryopreservation (24 h, 1, 6 and 12 months) on sperm motility, viability, DNA integrity, ATP content, total antioxidant capacity (TAC), morphology and sperm ultrastructure in this species. Milt samples from six males were cryopreserved in a medium containing final concentrations of 7.5% Me2SO, 4.1% glucose and 9.0% egg yolk. The samples were frozen in liquid nitrogen (LN) vapor and stored in LN for periods of 24 h and 1, 6 and 12 months. After thawing, both the motility rate and the viability decreased significantly compared with fresh sperm; however, these parameters did not differ among the four cryopreservation times. The DNA integrity and ATP content decreased significantly after 6 months of cryopreservation. There were no significant differences in TAC values between fresh and cryopreserved sperm. The total sperm abnormalities in cryopreserved samples were about 5-fold higher than in fresh sperm; short tail was the most common defect occurring after cryostorage. The ultrastructural analysis reveals that P. orinoquensis spermatozoa consist of an ovoid head without acrosome, a cylindrical mid-piece, and a single flagellum. The nuclear fossa is located at the base of the nucleus and contains the centriolar complex. There are 1-2 ring-shaped mitochondria located in the mid-piece. The flagellum shows a 9 + 2 organization of microtubules in the axoneme. Post-thaw spermatozoa presented damage such as swelling and rupture of the plasma membrane, mitochondrial damage, loss of the electron-dense chromatin of the nucleus, and degeneration in the middle region.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5343
dc.language.isoen
dc.publisherELSEVIER
dc.sourceAQUACULTURE REPORTS
oaire.resourceTypeArticle
uct.indizacionSCI
Files
Collections
MigraciĆ³n Web of Science