Cryostorage of white cachama (Piaractus orinoquensis) sperm: Effects on cellular, biochemical and ultrastructural parameters
Cryostorage of white cachama (Piaractus orinoquensis) sperm: Effects on cellular, biochemical and ultrastructural parameters
Authors
Medina Robles, Victor Mauricio
Sandoval Vargas, Leydy Yasmin
Suarez Martinez, Roger Oswaldo
Gomez Ramirez, Edwin
Guaje Ramirez, Diana Nataly
Cruz Casallas, Pablo Emilio
Sandoval Vargas, Leydy Yasmin
Suarez Martinez, Roger Oswaldo
Gomez Ramirez, Edwin
Guaje Ramirez, Diana Nataly
Cruz Casallas, Pablo Emilio
Profesor GuĆa
Authors
Date
Datos de publicaciĆ³n:
10.1016/j.aqrep.2023.101477
AQUACULTURE REPORTS,Vol.29,,2023
AQUACULTURE REPORTS,Vol.29,,2023
Tipo de recurso
Article
Keywords
Materia geogrƔfica
Collections
Abstract
To date, little attention has been paid to identifying the effects of long-term cryopreservation on sperm quality for Piaractus orinoquensis. The object of this study was therefore to evaluate the effect of long-term cryopreservation (24 h, 1, 6 and 12 months) on sperm motility, viability, DNA integrity, ATP content, total antioxidant capacity (TAC), morphology and sperm ultrastructure in this species. Milt samples from six males were cryopreserved in a medium containing final concentrations of 7.5% Me2SO, 4.1% glucose and 9.0% egg yolk. The samples were frozen in liquid nitrogen (LN) vapor and stored in LN for periods of 24 h and 1, 6 and 12 months. After thawing, both the motility rate and the viability decreased significantly compared with fresh sperm; however, these parameters did not differ among the four cryopreservation times. The DNA integrity and ATP content decreased significantly after 6 months of cryopreservation. There were no significant differences in TAC values between fresh and cryopreserved sperm. The total sperm abnormalities in cryopreserved samples were about 5-fold higher than in fresh sperm; short tail was the most common defect occurring after cryostorage. The ultrastructural analysis reveals that P. orinoquensis spermatozoa consist of an ovoid head without acrosome, a cylindrical mid-piece, and a single flagellum. The nuclear fossa is located at the base of the nucleus and contains the centriolar complex. There are 1-2 ring-shaped mitochondria located in the mid-piece. The flagellum shows a 9 + 2 organization of microtubules in the axoneme. Post-thaw spermatozoa presented damage such as swelling and rupture of the plasma membrane, mitochondrial damage, loss of the electron-dense chromatin of the nucleus, and degeneration in the middle region.