A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage

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dc.contributor.authorBeltran, Jorge F.
dc.contributor.authorZamorano, Mauricio
dc.contributor.authorHerrera Belen, Lisandra
dc.contributor.authorRisopatron, Jennie
dc.contributor.authorValdebenito, Ivan
dc.contributor.authorNorambuena, Juan-Alejandro
dc.contributor.authorFarias, Joaquin A.
dc.contributor.authorFarias, Jorge G.
dc.date2021
dc.date.accessioned2021-10-04T18:44:48Z
dc.date.available2021-10-04T18:44:48Z
dc.description.abstractSperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of this cation have also been shown to be one of the most important factors affecting motility in cold-stored sperm cells. Taking into account the aforementioned aspects, we carried out a differential proteomic study on Salmo salar sperm stored at 4 degrees C with the aim of evaluating the protein profile on day 0 and day 14. The results of our study showed that 401 proteins were significantly downregulated (p < 0.05) on day 14, where four of them are key in the sperm motility of Salmo salar. The results of this study will allow a better understanding of the sperm activation mechanisms of Salmo salar, which will be of great importance in the design of future cold storage strategies for sperm preservation.
dc.identifier.citationAQUACULTURE,Vol.545,,2021
dc.identifier.doi10.1016/j.aquaculture.2021.737211
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/4241
dc.language.isoen
dc.publisherELSEVIER
dc.sourceAQUACULTURE
dc.subject.englishSalmo salar
dc.subject.englishMotility
dc.subject.englishProteomics
dc.subject.englishChannel
dc.subject.englishCalcium
dc.titleA differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage
dc.typeArticle
uct.indizacionSCI
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