A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage

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Beltran, Jorge F.
Zamorano, Mauricio
Herrera Belen, Lisandra
Risopatron, Jennie
Valdebenito, Ivan
Norambuena, Juan-Alejandro
Farias, Joaquin A.
Farias, Jorge G.
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AQUACULTURE,Vol.545,,2021
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Abstract
Sperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of this cation have also been shown to be one of the most important factors affecting motility in cold-stored sperm cells. Taking into account the aforementioned aspects, we carried out a differential proteomic study on Salmo salar sperm stored at 4 degrees C with the aim of evaluating the protein profile on day 0 and day 14. The results of our study showed that 401 proteins were significantly downregulated (p < 0.05) on day 14, where four of them are key in the sperm motility of Salmo salar. The results of this study will allow a better understanding of the sperm activation mechanisms of Salmo salar, which will be of great importance in the design of future cold storage strategies for sperm preservation.
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