Fish sperm cryopreservation using biodegradable containers: New low-cost and environment-friendly methodology

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datacite.alternateIdentifier.citationREPRODUCTION,Vol.166,89-97,2023
datacite.alternateIdentifier.doi10.1530/REP-23-0046
datacite.creatorFranca, Thales de Souza
datacite.creatorGomes, Itamar Cossina
datacite.creatorSanches, Eduardo Antonio
datacite.creatorAtehortua, Maritza Perez
datacite.creatorTeixeira, Nathalia dos Santos
datacite.creatorRodrigues, Romulo Batista
datacite.creatorde Freitas, Thaiza Rodrigues
datacite.creatorBenato, Jhony Lisboa
datacite.creatorMarques, Lis Santos
datacite.creatorde Souza, Ana Regina Seabra
datacite.creatorStreit Jr, Danilo Pedro
datacite.date2023
datacite.titleFish sperm cryopreservation using biodegradable containers: New low-cost and environment-friendly methodology
dc.date.accessioned2024-05-27T18:26:06Z
dc.date.available2024-05-27T18:26:06Z
dc.description.abstractContainers used to cryopreserve sperm are made with non-biodegradable plastic compounds, having a high monetary and environmental cost. Therefore, the development of biodegradable alternative containers for cell cryopreservation is necessary. Thus, this study aimed to evaluate the efficiency of hard-gelatin and hard-hydroxypropyl methylcellulose (HPMC) capsules as low-cost and biodegradable alternative containers for sperm cryopreservation. Sperm from 12South American silver catfish Rhamdia quelen were individually cryopreserved in plastic straws 0.25 mL (as control), hard-gelatin, and hard-HPMC capsules. The quality of post-thaw sperm cryopreserved in the different containers was checked by measuring spermatozoa membrane integrity, kinetic parameters, mitochondrial activity, fertilization, hatching, and normal larvae rates. The samples cryopreserved in straws showed a higher percentage of membrane integrity (68%) than those frozen in hard-gelatin (40%) and hard-HPMC capsules (40%). However, we did not observe differences between the samples stored in straws and hard capsules for the rest of the tested sperm parameters. Thus, based on the high sperm fertility capability, both capsules were efficient as cryopreservation containers for maintaining sperm functionality.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5671
dc.language.isoen
dc.publisherBIOSCIENTIFICA LTD
dc.sourceREPRODUCTION
oaire.resourceTypeArticle
uct.indizacionSCI
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