Fish sperm cryopreservation using biodegradable containers: New low-cost and environment-friendly methodology
Fish sperm cryopreservation using biodegradable containers: New low-cost and environment-friendly methodology
Authors
Franca, Thales de Souza
Gomes, Itamar Cossina
Sanches, Eduardo Antonio
Atehortua, Maritza Perez
Teixeira, Nathalia dos Santos
Rodrigues, Romulo Batista
de Freitas, Thaiza Rodrigues
Benato, Jhony Lisboa
Marques, Lis Santos
de Souza, Ana Regina Seabra
Streit Jr, Danilo Pedro
Gomes, Itamar Cossina
Sanches, Eduardo Antonio
Atehortua, Maritza Perez
Teixeira, Nathalia dos Santos
Rodrigues, Romulo Batista
de Freitas, Thaiza Rodrigues
Benato, Jhony Lisboa
Marques, Lis Santos
de Souza, Ana Regina Seabra
Streit Jr, Danilo Pedro
Profesor GuĆa
Authors
Date
Datos de publicaciĆ³n:
10.1530/REP-23-0046
REPRODUCTION,Vol.166,89-97,2023
REPRODUCTION,Vol.166,89-97,2023
Tipo de recurso
Article
Keywords
Materia geogrƔfica
Collections
Abstract
Containers used to cryopreserve sperm are made with non-biodegradable plastic compounds, having a high monetary and environmental cost. Therefore, the development of biodegradable alternative containers for cell cryopreservation is necessary. Thus, this study aimed to evaluate the efficiency of hard-gelatin and hard-hydroxypropyl methylcellulose (HPMC) capsules as low-cost and biodegradable alternative containers for sperm cryopreservation. Sperm from 12South American silver catfish Rhamdia quelen were individually cryopreserved in plastic straws 0.25 mL (as control), hard-gelatin, and hard-HPMC capsules. The quality of post-thaw sperm cryopreserved in the different containers was checked by measuring spermatozoa membrane integrity, kinetic parameters, mitochondrial activity, fertilization, hatching, and normal larvae rates. The samples cryopreserved in straws showed a higher percentage of membrane integrity (68%) than those frozen in hard-gelatin (40%) and hard-HPMC capsules (40%). However, we did not observe differences between the samples stored in straws and hard capsules for the rest of the tested sperm parameters. Thus, based on the high sperm fertility capability, both capsules were efficient as cryopreservation containers for maintaining sperm functionality.