Persistence and pathway of glyphosate degradation in the coastal wetland soil of central Delaware

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datacite.alternateIdentifier.citationJOURNAL OF HAZARDOUS MATERIALS,Vol.477,2024
datacite.alternateIdentifier.doi10.1016/j.jhazmat.2024.135238
datacite.creatorMoller, Spencer R.
datacite.creatorCampos, Marco A.
datacite.creatorRilling, Joaquin I.
datacite.creatorBakkour, Rani
datacite.creatorHollenback, Anthony J.
datacite.creatorJorquera, Milko A.
datacite.creatorJaisi, Deb P.
datacite.date2024
datacite.subject.englishAminomethylphosphonic acid (AMPA)
datacite.subject.englishUHPLC
datacite.subject.englishOrbitrap MS
datacite.subject.englishDegradation pathway
datacite.subject.englishGene expression
datacite.subject.englishGene abundance
datacite.subject.englishPhnJ gene
datacite.titlePersistence and pathway of glyphosate degradation in the coastal wetland soil of central Delaware
dc.date.accessioned2024-09-10T18:47:09Z
dc.date.available2024-09-10T18:47:09Z
dc.description.abstractGlyphosate is a globally dominant herbicide. Here, we studied the degradation and microbial response to glyphosate application in a wetland soil in central Delaware for controlling invasive species (Phragmites australis). We applied a two-step solid-phase extraction method using molecularly imprinted polymers designed for the separation and enrichment of glyphosate and aminomethylphosphonic acid (AMPA) from soils before their analysis by ultra-high-performance liquid chromatography (UHPLC) and Q Exactive Orbitrap mass spectrometry methods. Our results showed that approximately 90 % of glyphosate degraded over 100 d after application, with AMPA being a minor (<10 %) product. Analysis of glyphosate-specific microbial genes to identify microbial response and function revealed that the expression of the phnJ gene, which codes C-P lyase enzyme, was consistently dominant over the gox gene, which codes glyphosate oxidoreductase enzyme, after glyphosate application. Both gene and concentration data independently suggested that C-P bond cleavage-which forms sarcosine or glycine-was the dominant degradation pathway. This is significant because AMPA, a more toxic product, is reported to be the preferred pathway of glyphosate degradation in other soil and natural environments. The degradation through a safer pathway is encouraging for minimizing the detrimental impacts of glyphosate on the environment.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5931
dc.language.isoen
dc.publisherELSEVIER
dc.sourceJOURNAL OF HAZARDOUS MATERIALS
oaire.resourceTypeArticle
uct.indizacionSCI
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