Glutathion peroxidase activity (GSH-Px) in grazing dairy cattle in the south of Chile (IXth Region) and their relation with selenium contents in the forage
Glutathion peroxidase activity (GSH-Px) in grazing dairy cattle in the south of Chile (IXth Region) and their relation with selenium contents in the forage
Authors
Wittwer, F.
Araneda, P.
Ceballos, A.
Contreras, P.A.
Andaur Rademacher, Marcela
Bohmwald, H.
Araneda, P.
Ceballos, A.
Contreras, P.A.
Andaur Rademacher, Marcela
Bohmwald, H.
Authors
Date
2012-02-24
Datos de publicación:
Archivos de Medicina Veterinaria, Vol.34, N°1, 49-57, 2002
Keywords
Selenio - Bovino - Forraje
Collections
Abstract
Con el objeto de determinar la actividad sanguínea de glutatión peroxidasa (GSH-Px; E.C. 1.11.1.9) en bovinos lecheros de la IX Región de la Araucanía (38º LS y 71º LO), Chile, y establecer su eventual asociación con el contenido de Se en el forraje, durante el otoño y la primavera de 1999 se tomaron muestras de forraje de las praderas y de sangre en grupos de vacas en inicio de lactancia y vaquillas en 12 predios lecheros. Se midió la concentración de Se en el forraje empleando la técnica de espectroscopía de plasma acoplado inductivamente con detector de masas (ICP-MS) y la actividad sanguínea de GSH-Px mediante una técnica cinética compuesta NADPH-dependiente. La concentración de Se del forraje fue 0.03±0.02 ppm, donde un 83% presentó valores deficitarios (<0,05 ppm). La actividad de GSH-Px fue 129±112 U/g Hb, encontrando en las vacas una mayor actividad que en las vaquillas (p<0.05), con valores similares en otoño y primavera (p>0.05). Se encontró un 20% de los grupos analizados con valores deficitarios (< 60 U/g Hb) para la actividad enzimática. Se observó una asociación entre la actividad sanguínea de GSH-Px en vaquillas con el contenido del mineral en el forraje (r=0.74; p<0.05). Los resultados permiten concluir que en la mayoría de los forrajes producidos por las praderas del área lechera de la IX Región de la Araucanía presentan bajas concentraciones de Se para ser empleados en la alimentación de bovinos de lechería y su consumo se asocia a una baja actividad sanguínea de GSH-Px que señala deficiencias metabólicas de Se en los animales, viéndose mayormente afectadas las vaquillas tanto en otoño como en primavera
The content of selenium (Se) in forage samples and the blood activity of glutathione peroxidase (GSH-Px; EC 1.11.1.9) of groups of lactating cows and heifers were evaluated between autumn and spring of 1999 in 12 dairy herds from IXth Region (38º S and 71º W) of Chile. Se-concentration was determined in forage samples by using inductively coupled plasma mass spectroscopy (ICP-MS). The activity of GSH-Px was determined through a kinetic technique NADPH-dependant. Mean, standard deviation and range were obtained, and the groups were compared by ANOVA test and the values compared to reference values. The content of Se in forage samples was 0.03±0.02 ppm, the range was between < 0.01 to 0.08 ppm. 83% of the samples had a Se-concentration below the minimum dietary requirements (<0.05 ppm) for grazing cattle. Blood activity of GSH-Px was 129±112 U/g Hb, and the range was 34-545 U/g Hb. There was not difference between autumn and spring (p>0.05). Heifers showed a lower activity for GSH-Px than cows in both periods (p<0.05). Twenty percent of the animals showed a low activity of GSH-Px (below 60 U/g Hb), meaning a Se deficiency in the animals. There was a relation between the content of Se in forage and the blood activity of GSH-Px in heifers (r= 0.74; p<0.05). Accordingly, the content of Se in most of the forage produced in the dairy cattle area from the IXth Region, Chile is below the nutritional requirements for grazing dairy cattle and nutritional metabolic deficiencies of Se are present in dairy herds according to blood activity of GSH-Px. Heifers are mostly affected
The content of selenium (Se) in forage samples and the blood activity of glutathione peroxidase (GSH-Px; EC 1.11.1.9) of groups of lactating cows and heifers were evaluated between autumn and spring of 1999 in 12 dairy herds from IXth Region (38º S and 71º W) of Chile. Se-concentration was determined in forage samples by using inductively coupled plasma mass spectroscopy (ICP-MS). The activity of GSH-Px was determined through a kinetic technique NADPH-dependant. Mean, standard deviation and range were obtained, and the groups were compared by ANOVA test and the values compared to reference values. The content of Se in forage samples was 0.03±0.02 ppm, the range was between < 0.01 to 0.08 ppm. 83% of the samples had a Se-concentration below the minimum dietary requirements (<0.05 ppm) for grazing cattle. Blood activity of GSH-Px was 129±112 U/g Hb, and the range was 34-545 U/g Hb. There was not difference between autumn and spring (p>0.05). Heifers showed a lower activity for GSH-Px than cows in both periods (p<0.05). Twenty percent of the animals showed a low activity of GSH-Px (below 60 U/g Hb), meaning a Se deficiency in the animals. There was a relation between the content of Se in forage and the blood activity of GSH-Px in heifers (r= 0.74; p<0.05). Accordingly, the content of Se in most of the forage produced in the dairy cattle area from the IXth Region, Chile is below the nutritional requirements for grazing dairy cattle and nutritional metabolic deficiencies of Se are present in dairy herds according to blood activity of GSH-Px. Heifers are mostly affected