Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)
Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)
Authors
Jara Seguel, Pedro
Valdebenito Isler, Iván
Palma Rojas, Claudio
Rebolledo, Christian
Valdebenito Isler, Iván
Palma Rojas, Claudio
Rebolledo, Christian
Authors
Date
2012-02-24
Datos de publicación:
10.4067/S0718-560X2008000100007
Keywords
Galaxias maculatus - ADN nuclear
Collections
Abstract
El contenido de ADN nuclear (valor 2C) fue determinado en el pez comercial Galaxias maculatus (Galaxiidae) usando microdensitometría de núcleos de eritrocitos sometidos a tinción de Feulgen, utilizando como estándar eritrocitos de trucha arco iris con un valor 2C conocido. El valor 2C de G. maculatus fue 2,21 ± 0,12 pg y su valor C es equivalente a 1,105 pg (1.082,9 pMb). Este valor C está dentro del rango registrado para otras especies de osmeriformes (0,62-3,2 pg). El diámetro promedio de la cabeza del espermatozoide de G. maculatus es menor al promedio descrito para la trucha arco iris utilizado como estándar, lo que coincide con las diferencias observadas en el contenido de ADN nuclear entre ambas especies. Estos datos contribuyen a ampliar los antecedentes genómicos disponibles para G. maculatus y podrían ser útiles en futuros programas tendientes a su manipulación genética.
The nuclear DNA content (2C value) was determined in the commercial fish Galaxias maculatus (Galaxiidae) was determined by microdensitometry of erythrocyte nuclei after Feulgen staining; rainbow trout erythrocytes with a known 2C value were used as a standard. The 2C value of G. maculatus was 2.21 ± 0.12 pg and its C value was equivalent to 1.105 pg (1,082.9 Mbp). This C value is within the range recorded for other osmeriform species (0.62-3.2 pg). The average sperm head diameter of G. maculatus is lower than the average sperm head diameter of rainbow trout (used as a standard), which coincides with the differences observed in the nuclear DNA content of both species. This information increases the genome data available for G. maculatus and might be useful in future programs dealing with its genetic manipulation.
The nuclear DNA content (2C value) was determined in the commercial fish Galaxias maculatus (Galaxiidae) was determined by microdensitometry of erythrocyte nuclei after Feulgen staining; rainbow trout erythrocytes with a known 2C value were used as a standard. The 2C value of G. maculatus was 2.21 ± 0.12 pg and its C value was equivalent to 1.105 pg (1,082.9 Mbp). This C value is within the range recorded for other osmeriform species (0.62-3.2 pg). The average sperm head diameter of G. maculatus is lower than the average sperm head diameter of rainbow trout (used as a standard), which coincides with the differences observed in the nuclear DNA content of both species. This information increases the genome data available for G. maculatus and might be useful in future programs dealing with its genetic manipulation.