Effects of type of substrate and dilution rate on fermentation in serial rumen mixed cultures
Date
Authors
Ungerfeld, Emilio Mauricio
Cancino-Padilla, Nathaly
Vera-Aguilera, Nelson
Scorcione, María Carolina
Saldivia, Marcelo
Lagos-Pailla, Lorena
Vera, Milena
Cerda, Cristián
Muñoz, Camila
Urrutia, Natalie L.
Cancino-Padilla, Nathaly
Vera-Aguilera, Nelson
Scorcione, María Carolina
Saldivia, Marcelo
Lagos-Pailla, Lorena
Vera, Milena
Cerda, Cristián
Muñoz, Camila
Urrutia, Natalie L.
Authors
Date
Datos de publicación:
10.3389/fmicb.2024.1356966
Keywords
Dihydrogen - Dilution Rate - Lactate - Methane - Methanogens - Rumen Fermentation - Type Of Substrate - Volatile Fatty Acids - Carbon 13 - Hydrogen Peroxide - Lactic Acid - Pyruvic Acid - 000 - 60/80 Carboxen 1 - Clarus 580 Perkin Elmer Gc - Illumina Nextera Xt Index Kit - Kromasil Rp-18e - Lc-20a Shimadzu Hplc - Maestrogen Spectrophotometer - Orion Star A214 - Qubit Dsdna Hs Assay Kit - Shimadzu Qp2010 Plus - Shimadzu Spd-m20a Photodiode Array Detector - Carbon 13 - Hydrogen Peroxide - Isotope - Lactic Acid - Organic Matter - Pyruvic Acid - Rna 16s - Volatile Fatty Acid - Article - Bacterium Culture - Biomass - Cell Culture - Decarboxylation - Dna Extraction - Enzyme Substrate - Fermentation - Fluorometry - High Performance Liquid Chromatography - Incubation Time - Mass Fragmentography - Methanogen - Methanogenesis - Nonhuman - Ph - Real Time Polymerase Chain Reaction - Rumen - Thermodynamics
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Abstract
Forages and concentrates have consistently distinct patterns of fermentation in the rumen, with forages producing more methane (CH?) per unit of digested organic matter (OM) and higher acetate to propionate ratio than concentrates. A mechanism based on the Monod function of microbial growth has been proposed to explain the distinct fermentation pattern of forages and concentrates, where greater dilution rates and lower pH associated with concentrate feeding increase dihydrogen (H?) concentration through increasing methanogens growth rate and decreasing methanogens theoretically maximal growth rate, respectively. Increased H? concentration would in turn inhibit H? production, decreasing methanogenesis, inhibit H?-producing pathways such as acetate production via pyruvate oxidative decarboxylation, and stimulate H?-incorporating pathways such as propionate production.
Description
Keywords
Dihydrogen , Dilution Rate , Lactate , Methane , Methanogens , Rumen Fermentation , Type Of Substrate , Volatile Fatty Acids , Carbon 13 , Hydrogen Peroxide , Lactic Acid , Pyruvic Acid , 000 , 60/80 Carboxen 1 , Clarus 580 Perkin Elmer Gc , Illumina Nextera Xt Index Kit , Kromasil Rp-18e , Lc-20a Shimadzu Hplc , Maestrogen Spectrophotometer , Orion Star A214 , Qubit Dsdna Hs Assay Kit , Shimadzu Qp2010 Plus , Shimadzu Spd-m20a Photodiode Array Detector , Carbon 13 , Hydrogen Peroxide , Isotope , Lactic Acid , Organic Matter , Pyruvic Acid , Rna 16s , Volatile Fatty Acid , Article , Bacterium Culture , Biomass , Cell Culture , Decarboxylation , Dna Extraction , Enzyme Substrate , Fermentation , Fluorometry , High Performance Liquid Chromatography , Incubation Time , Mass Fragmentography , Methanogen , Methanogenesis , Nonhuman , Ph , Real Time Polymerase Chain Reaction , Rumen , Thermodynamics
Citation
10.3389/fmicb.2024.1356966