Efectos del ácido giberélico, bencilaminopurina y fluridona en la germinación in vitro de Ugni molinae Turcz. (Myrtaceae)
Efectos del ácido giberélico, bencilaminopurina y fluridona en la germinación in vitro de Ugni molinae Turcz. (Myrtaceae)
Authors
Rodríguez Beraud, Mario
Hormazábal Vásquez, Nelson
Araneda Durán, Ximena
Tampe Pérez, Jocelyne
Lobos Marambio, Victoria
Castillo Rubio, Claudia
Hormazábal Vásquez, Nelson
Araneda Durán, Ximena
Tampe Pérez, Jocelyne
Lobos Marambio, Victoria
Castillo Rubio, Claudia
Authors
Date
2016
Datos de publicación:
10.4067/S0717-66432016000100010
Keywords
Mirtáceas - Ácido abscísico - Dormancia - Murtilla
Collections
Abstract
En la presente investigación se estudiaron los efectos de bencilaminopurina (BAP), ácido giberélico (GA ) y fluridona (FLU) sobre la germinación in vitro de murtilla (Ugni molinae), una especie nativa de Chile. La semilla de murtilla presenta una fuerte dormancia, razón por la cual se realizaron dos ensayos a fin de mejorar la germinación. Los resultados indican que FLU, un inhibidor de la síntesis de ABA, fue el más eficaz en romper la dormancia. La combinación de 10 μM de FLU con BAP o GA mejoraron la germinación, siendo FLU + GA la más óptima, ésta fue capaz de elevar la germinación de 34% en el testigo a 96% a los 60 días. Lo anterior sugiere que en los mecanismos de dormancia de la semilla de U. molinae está involucrada la presencia de ABA.
In the present investigation, the effects of benzylaminopurine (BAP), gibberellic acid (GA3) and fluridone (FLU) on in-vitro germination of murtilla (Ugni molinae), a native species to Chile were studied. Murtilla seed has a strong dormancy, the reason why two trials were performed to improve the germination. The results show that FLU, an inhibitor of the synthesis of ABA, was the most effective in breaking dormancy. The combination of 10 μM FLU with BAP or GA3 improved germination, FLU + GA3 was the optimum combination. It raised the germination from 34% in the control seed to 96% up to 60 days after treatment. The results also suggest that in the mechanisms of seed dormancy of U. molinae the presence of ABA is involved.
In the present investigation, the effects of benzylaminopurine (BAP), gibberellic acid (GA3) and fluridone (FLU) on in-vitro germination of murtilla (Ugni molinae), a native species to Chile were studied. Murtilla seed has a strong dormancy, the reason why two trials were performed to improve the germination. The results show that FLU, an inhibitor of the synthesis of ABA, was the most effective in breaking dormancy. The combination of 10 μM FLU with BAP or GA3 improved germination, FLU + GA3 was the optimum combination. It raised the germination from 34% in the control seed to 96% up to 60 days after treatment. The results also suggest that in the mechanisms of seed dormancy of U. molinae the presence of ABA is involved.