Aspergillus fumigatus-sensitive IgE is associated with bronchial hypersensitivity in a murine model of neutrophilic airway inflammation
Date
Authors
ORTLOFF TRAUTMANN, ALEXANDER
Morán, Gabriel
Uberti, Benjamin
Ortloff, Alexander R.
Folch, Hugo
Morán, Gabriel
Uberti, Benjamin
Ortloff, Alexander R.
Folch, Hugo
Authors
Date
Datos de publicación:
10.1016/j.mycmed.2017.11.005
Keywords
Aspergillus Fumigatus - Ige - Neutrophilic Asthma - Immunoglobulin E - Fungal Proteins - Immunoglobulin E - Fungal Extract - Fungal Protein - Immunoglobulin E - Immunoglobulin E Antibody - Animal Cell - Animal Experiment - Animal Model - Animal Tissue - Article - Aspergillosis - Aspergillus Fumigatus - Asthma - Bronchial Hypersensitivity - Bronchoalveolar Lavage Fluid - Cell Activation - Controlled Study - Cytology - Disease Association - Disease Severity - Enzyme Linked Immunosorbent Assay - Histology - Lung Parenchyma - Mast Cell Degranulation - Mouse - Neutrophil - Neutrophilic Airway Inflammation - Nonhuman - Protein Function - Rat - Respiratory Tract Allergy - Respiratory Tract Inflammation - Animal - Bagg Albino Mouse - Bioassay - Bronchus Hyperreactivity - Chemistry - Disease Model - Female - Fungus Spore - Hypersensitivity - Immunology - Inflammation - Lung - Mast Cell - Microbiology - Pathology - Pathophysiology - Animals - Asthma - Biological Assay - Bronchial Hyperreactivity - Bronchoalveolar Lavage Fluid - Disease Models, Animal - Female - Fungal Proteins - Hypersensitivity - Immunoglobulin E - Inflammation - Lung - Mast Cells - Mice - Mice, Inbred Balb C - Neutrophils - Spores, Fungal
Collections
Abstract
Neutrophils are the predominant inflammatory cells that infiltrate airways during acute exacerbation of asthma. The importance of A. fumigatus sensitization, and IgE response in the airways in patients with acute asthma is unclear. Rockefeller (RK) mice were sensitized with A. fumigatus extract protein. The animals were subsequently challenged with different degrees of A. fumigatus contamination in the cage bedding. All groups of mice were euthanized to obtain bronchoalveolar lavage fluid (BALF) for cytological and Elisa assays, and lung tissue for histological analysis. Moreover, several bioassays were conducted to determine whether BALF IgE antibodies can activate mast cells. In this study, we demonstrated that exposure of sensitized mice to a known concentration of A. fumigatus conidia produces bronchial hyperreactivity with marked neutrophilic bronchial infiltration and increased BALF IgE, capable of triggering mast cell degranulation. This study suggests that IgE may play a role in bronchial hyperreactivity associated to A. fumigatus exposure in mice. Mice sensitized and challenged with this fungus showed characteristics of severe asthma, with an increase of BALF neutrophils, histological changes consistent with severe asthma and an increase of IgE capable of triggering type I hypersensitivity. © 2019 Elsevier B.V., All rights reserved.
Description
Keywords
Aspergillus Fumigatus , Ige , Neutrophilic Asthma , Immunoglobulin E , Fungal Proteins , Immunoglobulin E , Fungal Extract , Fungal Protein , Immunoglobulin E , Immunoglobulin E Antibody , Animal Cell , Animal Experiment , Animal Model , Animal Tissue , Article , Aspergillosis , Aspergillus Fumigatus , Asthma , Bronchial Hypersensitivity , Bronchoalveolar Lavage Fluid , Cell Activation , Controlled Study , Cytology , Disease Association , Disease Severity , Enzyme Linked Immunosorbent Assay , Histology , Lung Parenchyma , Mast Cell Degranulation , Mouse , Neutrophil , Neutrophilic Airway Inflammation , Nonhuman , Protein Function , Rat , Respiratory Tract Allergy , Respiratory Tract Inflammation , Animal , Bagg Albino Mouse , Bioassay , Bronchus Hyperreactivity , Chemistry , Disease Model , Female , Fungus Spore , Hypersensitivity , Immunology , Inflammation , Lung , Mast Cell , Microbiology , Pathology , Pathophysiology , Animals , Asthma , Biological Assay , Bronchial Hyperreactivity , Bronchoalveolar Lavage Fluid , Disease Models, Animal , Female , Fungal Proteins , Hypersensitivity , Immunoglobulin E , Inflammation , Lung , Mast Cells , Mice , Mice, Inbred Balb C , Neutrophils , Spores, Fungal
Citation
10.1016/j.mycmed.2017.11.005