Effects of UV-B radiation on anatomical characteristics, phenolic compounds and gene expression of the phenylpropanoid pathway in highbush blueberry leaves
Date
Authors
INOSTROZA BLANCHETEAU, CLAUDIO ANDRES
Inostroza-Blancheteau, Claudio
Reyes-Diaz, Marjorie
Arellano, Alejandro
Latsague, Mirtha
Acevedo, Patricio
Loyola, Rodrigo
Arce-Johnson, Patricio
Alberdi, Miren
Inostroza-Blancheteau, Claudio
Reyes-Diaz, Marjorie
Arellano, Alejandro
Latsague, Mirtha
Acevedo, Patricio
Loyola, Rodrigo
Arce-Johnson, Patricio
Alberdi, Miren
Authors
Date
Datos de publicación:
10.1016/j.plaphy.2014.10.015
Keywords
Blueberry - Lipid peroxidation - MYBPA1 transcription factor - Phenolic compounds - UV-B radiation
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Abstract
The effects of increased doses of UV-B radiation on anatomical, biochemical and molecular features of leaves of two highbush blueberry (Vaccinium colymbosum L cv. Brigitta and Bluegold) genotypes were investigated. Plants were grown in a solid substrate and exposed to 0, 0.07, 0.12 and 0.19 Wm(-2) of biologically effective UV-B radiation for up to 72 h. Leaf thickness and the adaxial epidermis thickness fell more than 3-fold in both genotypes at the highest UV-B dose. Moreover, in Bluegold an evident disorganization in the different cell layers was observed at the highest UV-B radiation. A significant decrease in chlorophyll a/b after 6 h in Brigitta under the greater UV-B doses was observed. Anthocyanin and total phenolics were increased, especially at 0.19 Wm(-2), when compared to the control in both genotypes. Chlorogenic acid was the most abundant hydroxycinnamic acid in Brigitta, and was significantly higher (P <= 0.05) than in Bluegold leaves. Regarding the expression of phenylpropanoid genes, only the transcription factor VcMYBPA1 showed a significant and sustained induction at higher doses of UV-B radiation in both genotypes compared to the controls. Thus, the reduction of leaf thickness concomitant with a lower lipid peroxidation and rapid enhancement of secondary metabolites, accompanied by a stable induction of the VcMYBPA1 transcription factor suggest a better performance against UV-B radiation of the Brigitta genotype. (C) 2014 Elsevier Masson SAS. All rights reserved.
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Keywords
Blueberry , Lipid peroxidation , MYBPA1 transcription factor , Phenolic compounds , UV-B radiation
Citation
10.1016/j.plaphy.2014.10.015