, GARCIA SANHUEZA, CAMILO ANDRES, Segura, Camilo, Yañez, Osvaldo, Galdámez, Antonio, Tapia, Victoria, Núñez, Marco Tulio, Osorio-Román, Igor Orlando, García, Camilo, García-Beltrán, Olimpo

A new Rhodamine-based Turn On fluorescent probe (E)- 3?,6?-bis(diethylamino)-2-((2,5-dimethoxybenzylidene)amino)spiro[isoindoline-1,9?-xanthen]-3-one (WGB) was synthesized. Results show that WGB is selective for Cu2+ cations, forming a WGB-Cu2+ complex in a 2:1 stoichiometry, confirmed through density functional theory (DFT) electronic structure calculations and reactive molecular dynamics (MD) simulations. Theoretical calculations agreed with the experimental data. The detection limit of WGB-Cu+2 complex is 6.76 × 10-8 M. Preliminary studies employing epifluorescence microscopy demonstrate that Cu2+ can be imaged in neuroblastoma SH-SY5Y cells treated with WGB. © 2022 Elsevier B.V., All rights reserved.