Perforated Patch Clamp in Non-Neuronal Cells, the Model of Mammalian Sperm Cells
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Date
2012-04-26
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Date
2012-04-26
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Abstract
This chapter deals with the steps required to obtain perforated patch-clamp recordings from
mammalian sperm cells. (in spite of the fact that these cells) In spite of the fact that these cells are not electrically excitable, they possess a number of conductances due to their
expression of ion channels and even neurotransmitter-gated ion-channels, many of which remain to be explored functionally and structurally. Detailed methods for obtaining cells suitable for electrophysiological recordings and protocols to perform patch-clamp
recordings are outlined in the text.
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Editor: Fatima Shad Kaneez