Identification of bacteria in potential mutualism with toxic <i>Alexandrium catenella</i> in Chilean Patagonian fjords by <i>in vitro</i> and field monitoring
Identification of bacteria in potential mutualism with toxic <i>Alexandrium catenella</i> in Chilean Patagonian fjords by <i>in vitro</i> and field monitoring
Authors
Yarimizu, Kyoko
Mardones, Jorge I.
Paredes Mella, Javier
Perera, Ishara Uhanie
Fujiyoshi, So
Fuenzalida, Gonzalo
Acuna, Jacquelinne J.
Ruiz Gil, Tay
Campos, Marco
Rilling, Joaquin Ignacio
Calabrano Miranda, Pedro
Vilugron, Jonnathan
Espinoza Gonzalez, Oscar
Guzman, Leonardo
Nagai, Satoshi
Jorquera, Milko A.
Maruyama, Fumito
Mardones, Jorge I.
Paredes Mella, Javier
Perera, Ishara Uhanie
Fujiyoshi, So
Fuenzalida, Gonzalo
Acuna, Jacquelinne J.
Ruiz Gil, Tay
Campos, Marco
Rilling, Joaquin Ignacio
Calabrano Miranda, Pedro
Vilugron, Jonnathan
Espinoza Gonzalez, Oscar
Guzman, Leonardo
Nagai, Satoshi
Jorquera, Milko A.
Maruyama, Fumito
Profesor GuĆa
Authors
Date
Datos de publicaciĆ³n:
10.1371/journal.pone.0301343
PLOS ONE,Vol.19,2024
PLOS ONE,Vol.19,2024
Tipo de recurso
Article
Keywords
Materia geogrƔfica
Collections
Abstract
The dinoflagellate Alexandrium catenella is a well-known paralytic shellfish toxin producer that forms harmful algal blooms, repeatedly causing damage to Chilean coastal waters. The causes and behavior of algal blooms are complex and vary across different regions. As bacterial interactions with algal species are increasingly recognized as a key factor driving algal blooms, the present study identifies several bacterial candidates potentially associated with Chilean Alexandrium catenella. This research narrowed down the selection of bacteria from the Chilean A. catenella culture using antibiotic treatment and 16S rRNA metabarcoding analysis. Subsequently, seawater from two Chilean coastal stations, Isla Julia and Isla San Pedro, was monitored for two years to detect Alexandrium species and the selected bacteria, utilizing 16S and 18S rRNA gene metabarcoding analyses. The results suggested a potential association between Alexandrium species and Spongiibacteraceae at both stations. The proposed candidate bacteria within the Spongiibacteraceae family, potentially engaging in mutualistic relationships with Alexandrium species, included the genus of BD1-7 clade, Spongiibbacter, and Zhongshania.