Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification

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datacite.alternateIdentifier.citationTHERIOGENOLOGY,Vol.83,238-245,2015
datacite.alternateIdentifier.doi10.1016/j.theriogenology.2014.09.015
datacite.creatorFigueroa Villalobos, Elías
datacite.creatorMerino, O.
datacite.creatorRisopatron, J.
datacite.creatorIsachenko, V.
datacite.creatorSanchez, R.
datacite.creatorEffer Roldan, Brian
datacite.creatorIsachenko, E.
datacite.creatorFarias, J. G.
datacite.creatorValdebenito Isler, Iván
datacite.date2015
datacite.subject.englishSpermatozoa vitrification
datacite.subject.englishSalmo salar
datacite.subject.englishSeminal plasma
datacite.subject.englishSpermatozoa functionality
datacite.titleEffect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification
dc.date.accessioned2021-04-30T16:59:11Z
dc.date.available2021-04-30T16:59:11Z
dc.description.abstractThis study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0.13-M sucrose + SP at concentrations of 30% (G30), 40% (G40), or 50% (G50). Fresh sperm was used as a control. To freeze the samples, 30-mu L suspensions of spermatozoa from each group were dropped directly into liquid nitrogen. The resulting spheres were placed in cryotubes for storage in liquid nitrogen. The cryotubes with the vitrified spermatozoa were thawed by placing them in a water bath at 37 degrees C for 45 seconds. After thawing, the following sperm quality parameters were determined by flow cytometry: DNA fragmentation (terminal deoxynucleotidyl transferase dUTP nick end labeling), plasma membrane integrity (SYBR-14/PI, staining technique), and mitochondrial membrane potential (JC-1 staining). An optical microscope was used to assess subjectively sperm motility, whereas fertility was determined by the presence of neurulation using five replicates per treatment in a sample of 30 eggs. Spermatozoa quality variables were preserved best when the highest concentration of SP (50%) was used (DNA fragmentation, 9.2%; plasma membrane integrity, 98.6%; mitochondrial membrane integrity, 47.2%; motility, 44.1%; and fertility, 46.2%). (C) 2015 Elsevier Inc. All rights reserved.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3702
dc.language.isoen
dc.publisherELSEVIER SCIENCE INC
dc.sourceTHERIOGENOLOGY
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
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