Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)
Date
Authors
FIGUEROA VILLALOBOS, ELIAS GUSTAVO
VALDEBENITO ISLER, NEMESIO IVAN
Ulloa-Rodriguez, Patricio
Contreras, Pablo
Dumorne, Kelly
Figueroa, Elias
Risopatron, Jennie
Valdebenito, Ivan
Farias, Jorge G.
VALDEBENITO ISLER, NEMESIO IVAN
Ulloa-Rodriguez, Patricio
Contreras, Pablo
Dumorne, Kelly
Figueroa, Elias
Risopatron, Jennie
Valdebenito, Ivan
Farias, Jorge G.
Authors
Date
Datos de publicación:
10.1016/j.tice.2019.02.006
Keywords
Scanning electron microscopy - Transmission electron microscopy - Teleost - Fish spermatozoa
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Abstract
In this study, the morphology and ultrastructure of Eleginops maclovinus spermatozoa were characterized through scanning and electron microscopy. Findings revealed that E. maclovinus spermatozoa can be differentiated into three major parts: a spherical head without acrosome (typical for externally fertilizing teleost), a midpiece containing 2-5 spherical mitochondria, and a long flagellum. The mean length of the spermatozoa was 40.08 +/- 2.30 mu m, with flagella accounting for 38.38 +/- 2.08 mu m. The head was 1.31 +/- 0.17 mu m long, and 1.63 +/- 0.01 mu m wide. The midpiece was 0.39 +/- 0.05 mu m in length and 0.95 +/- 0.12 mu m in width. It was located below the nucleus and contained 2 to 5 spherical mitochondria. The mitochondria were separated from the axoneme by a cytoplasmic canal. There was no evidence of the flagellum membrane forming sidefins, and the axoneme was composed of the typical 9 + 2 microtubular doublet structure enclosed by cell membrane. The present study reveals that E. maclovinus sperm can be categorized as a primitive type. This study is the first to provide comprehensive details on the ultrastructure of spermatozoa in E. maclovinus.
Description
Keywords
Scanning electron microscopy , Transmission electron microscopy , Teleost , Fish spermatozoa
Citation
10.1016/j.tice.2019.02.006