Short-term storage sperm of coho salmon (Oncorhynchus kisutch) at 4 degrees C: Effect of sperm: Extender dilution ratios and antioxidant butyl-hydroxytoluene (BHT) on sperm function

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datacite.alternateIdentifier.citationCRYOBIOLOGY,Vol.95,44-50,2020
datacite.alternateIdentifier.doi10.1016/j.cryobiol.2020.06.007
datacite.creatorMerino, Osvaldo
datacite.creatorDumorne, Kelly
datacite.creatorSandoval Vargas, Leydy
datacite.creatorFigueroa Villalobos, Elías
datacite.creatorValdebenito Isler, Iván
datacite.creatorFarias, Jorge G.
datacite.creatorRisopatron, Jennie
datacite.date2020
datacite.subject.englishOncorhynchus kisutch
datacite.subject.englishSperm
datacite.subject.englishShort-term storage
datacite.subject.englishSperm: extender dilution
datacite.subject.englishButylated hydroxytoluene
datacite.subject.englishOxidative stress
datacite.titleShort-term storage sperm of coho salmon (Oncorhynchus kisutch) at 4 degrees C: Effect of sperm: Extender dilution ratios and antioxidant butyl-hydroxytoluene (BHT) on sperm function
dc.date.accessioned2021-04-30T16:40:38Z
dc.date.available2021-04-30T16:40:38Z
dc.description.abstractShort-term storage of semen is a necessary key procedure in fish; it allows maximizing the use of gametes. Nevertheless, sperm quality decreases during storage has been associated with oxidative stress damage due to an increase in reactive oxygen species (ROS) during storage. This study was designed to optimize a short-term storage protocol for Coho salmon (Oncorhynchus kisutch) spermatozoa, evaluating the effect of extender dilution and the addition of butyl-hydroxytoluene (BHT) antioxidant on sperm function parameters. In the first experiment, fresh semen was diluted in Storfish (R): extender dilution (1:2 and 1:3) and a control sample undiluted and stored at 4 degrees C for 7-days. In both experiments motility (MO), viability and integrity of plasma membrane, mitochondrial membrane potential (MMP) and superoxide anion level (O-2(-)) were evaluated at 0, 3 and 7 days. Result shows that, 1:3 dilution maintained a higher sperm function for a longer period time. In the second experiment, spermatozoa were suspended in Storfish (R) (1:3) supplemented with two different concentrations of BHT (1.0 mM and 2.0 mM) and a control sample without antioxidant and stored at 4 degrees C for 7 days. The results demonstrated that, antioxidant-supplemented samples greater MO than control samples (P < 0.05). The viability remained >75% during storage in all groups. MMP was higher in 2.0 mM BHT compared to 1.0 mM and control (P < 0.05), in addition, this concentration reduced O-2(-) level (P < 0.05). In conclusion, sperm: extender dilution 1:3 and adding of 2.0 mM BHT in sperm storage extender may enhance protection sperm function in Oncorhynchus kisutch against effects harmful of the oxidative stress during the in vitro storage.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3210
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.sourceCRYOBIOLOGY
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
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