Structure and genetic diversity of Canadian Maritimes wild hops
Structure and genetic diversity of Canadian Maritimes wild hops
Authors
Somalraju, Ashok
Soto Cerda, Braulio
Ghose, Kaushik
McCallum, Jason
Knox, Ron
Fofana, Bourlaye
Soto Cerda, Braulio
Ghose, Kaushik
McCallum, Jason
Knox, Ron
Fofana, Bourlaye
Profesor GuĆa
Authors
Date
Datos de publicaciĆ³n:
10.1139/gen-2023-0045
GENOME,Vol.67,24-30,2024
Tipo de recurso
WOS
Keywords
Materia geogrƔfica
Collections
Abstract
Studies on the northeastern American native hops (Humulus lupulus ssp. lupuloides) from the Canadian Maritimes are scarce. This study aimed to evaluate the genetic structure and diversity among 25 wild-collected hops from three Canadian Maritime provinces using microsatellite (simple sequence repeat (SSR)) markers. Based on 43 SSR markers, four distinct subgroups were found, with a low molecular variance (19%) between subgroups and a high variance (81%) within subgroups. The Nei's unbiased genetic distance between clusters ranged from 0.01 to 0.08, the genetic distance between clusters 2 and 3 being the farthest and that between clusters 1 and 2 the closest. Cluster 2 captured the highest overall diversity. A total of 18 SSR markers clearly discriminated hop clones by detecting putative subspecies-specific haplotypes, differentiating clones of native-wild H. lupulus ssp. lupuloides from the naturalized old and modern hop cultivars. Seven of the 18 SSR markers also differentiated two clones from the same site from one another. The study is the first, using molecular markers, to identify SSR markers with potential for intellectual property protection in Canadian Maritimes hops. The SSR markers herein used can be prime tools for hop breeders and growers in the region.