Effect of exogenous lipids on cryotolerance of Atlantic salmon (Salmo salar) spermatozoa

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datacite.alternateIdentifier.citationCRYOBIOLOGY,Vol.98,25-32,2021
datacite.alternateIdentifier.doi10.1016/j.cryobiol.2021.01.004
datacite.creatorDiaz, Rommy
datacite.creatorQuinones, John
datacite.creatorShort, Stefania
datacite.creatorContreras, Pablo
datacite.creatorUlloa Rodriguez, Patricio
datacite.creatorCancino Baier, David
datacite.creatorSepulveda, Nestor
datacite.creatorValdebenito Isler, Iván
datacite.creatorFarias, Jorge G.
datacite.date2021
datacite.subject.englishArachidonic acid
datacite.subject.englishAtlantic salmon
datacite.subject.englishFatty acids
datacite.subject.englishCholesterol
datacite.subject.englishSperm membrane
datacite.subject.englishFish spermatozoa
datacite.subject.englishFertility rates
datacite.titleEffect of exogenous lipids on cryotolerance of Atlantic salmon (Salmo salar) spermatozoa
dc.date.accessioned2021-04-30T17:04:08Z
dc.date.available2021-04-30T17:04:08Z
dc.description.abstractThe development of semen cryopreservation strategies is necessary to improve the semen storage technologies of species of great commercial interest for aquaculture. Recent studies demonstrate that lipids play an important role in the fertility and cryotolerance of fish gametes. This study investigated the effect of exogenous lipids in the freezing medium on the post-thaw functional parameters of Salmo solar spermatozoa. Semen samples (n = 12) were incubated in standard extender supplemented with different concentrations of oleic acid (OA, C18:1n9), linoleic acid (LA, C18:2n6), arachidonic acid (ARA, C20:4n6) and cholesterol-loaded cyclodextrin (CLC). Post-thaw motility, membrane integrity, mitochondrial membrane potential (Delta Psi m), superoxide anion (O-2(center dot-)) and fertility rates were analyzed. The results revealed that the semen incubated with 0.003 mmol/L OA increased the motility (similar to 7%) and Delta Psi m (similar to 2%) (P < 0.05), but membrane integrity and fertility were not increased. The addition of 0.003 mmol/L LA increased the motility (similar to 4%) and all LA extenders increased the Delta Psi m (P < 0.05); however, LA increased the (1) O-2(center dot-) levels and decreased the membrane integrity and fertility (P < 0.05). Semen incubated with ARA improved sperm motility (similar to 5%), membrane integrity (similar to 10.5%) and fertility rates (similar to 11%) (P < 0.05). The maximum improvement in post-thaw sperm functionality was observed by adding 0.003 mmol/L ARA. In contrast, sperm quality parameters and fertility were decreased by the CLC addition (P < 0.05). This study showed that ARA could be considered as an additive for semen cryopreservation and could be relevant in the reproductive process and reproductive management of Salmo solar.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3818
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.sourceCRYOBIOLOGY
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
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