In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas
| dc.contributor.author | Berland Olea, Marco | |
| dc.contributor.author | Baer, A. | |
| dc.contributor.author | Ruíz, J. | |
| dc.contributor.author | Parraguez, Víctor | |
| dc.contributor.author | Morales, P. | |
| dc.contributor.author | Adams, G.P. | |
| dc.contributor.author | Ratto Fuster, Marcelo | |
| dc.date | 2011 | |
| dc.date.accessioned | 2012-02-07T23:40:02Z | |
| dc.date.available | 2012-02-07T23:40:02Z | |
| dc.date.issued | 2012-02-07 | |
| dc.description.abstract | The objective was to evaluate the developmental competence of cumulus-oocyte complexes (COC) collected by follicular aspiration in llamas treated with FSH or eCG. Llamas were assigned randomly to two groups (n = 16 per group) and treated, at the time of ovarian follicular wave emergence, with either: 1) 25 mg of FSH im, twice daily for 4 d; or 2) 1000 IU of eCG as a single i.m. dose. The start of gonadotropin treatment was considered Day 0. Both groups were given 5 mg of Armour Standard LH im on Day 6, and COC were collected by follicle aspiration on Day 7. Expanded COC collected from FSH- (n = 157) and eCG-treated llamas (n = 151) were fertilized in vitro using epididymal sperm, and presumptive zygotes were in vitro cultured in SOF medium for 8 d. The FSH and eCG treatment groups did not differ with respect to: the number of follicles 7 mm (16.0; 2.7 vs 14.0; 1.9, respectively; P = 0.5); the number of COC collected (11.5; 1.9 vs 9.7; 1.2; P = 0.4); the number of expanded COC (9.8; 1.4 vs 9.4; 1.2; P = 0.8); or the percentage of presumptive zygotes which developed into 2 to 8 cell stage embryos (65.3 vs 63.1), morulas (46.2 vs 42.5), or blastocysts (23.1 vs 20.5; P > 0.05). In conclusion, FSH and eCG treatments were equally effective for recovery of a high number of expanded COC which were used directly for in vitro fertilization. Furthermore, rate of embryo development was not significantly affected by the gonadotropin treatment used. © 2011 Elsevier Inc. | es |
| dc.format | es | |
| dc.identifier.citation | Theriogenology, Vol. 75, N°8, 1482-1488, 2011 | es |
| dc.identifier.doi | 10.1016/j.theriogenology.2010.11.047 | es |
| dc.identifier.uri | https://repositoriodigital.uct.cl/handle/10925/589 | |
| dc.language.iso | en | es |
| dc.source | Theriogenology | es |
| dc.subject | Fertilización in vitro | es |
| dc.subject | Superestimulación | es |
| dc.subject | Gonadotropinas | es |
| dc.title | In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas | es |
| dc.type | Artículo de Revista | es |
| uct.carrera | Agronomía | es |
| uct.carrera | Ingeniería en Recursos Naturales Renovables | es |
| uct.carrera | Medicina Veterinaria | es |
| uct.catalogador | aga | es |
| uct.comunidad | Recursos Naturales | es |
| uct.facultad | Facultad de Recursos Naturales | es |
| uct.indizacion | ISI | es |