In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas

dc.contributor.authorBerland Olea, Marco
dc.contributor.authorBaer, A.
dc.contributor.authorRuíz, J.
dc.contributor.authorParraguez, Víctor
dc.contributor.authorMorales, P.
dc.contributor.authorAdams, G.P.
dc.contributor.authorRatto Fuster, Marcelo
dc.date2011
dc.date.accessioned2012-02-07T23:40:02Z
dc.date.available2012-02-07T23:40:02Z
dc.date.issued2012-02-07
dc.description.abstractThe objective was to evaluate the developmental competence of cumulus-oocyte complexes (COC) collected by follicular aspiration in llamas treated with FSH or eCG. Llamas were assigned randomly to two groups (n = 16 per group) and treated, at the time of ovarian follicular wave emergence, with either: 1) 25 mg of FSH im, twice daily for 4 d; or 2) 1000 IU of eCG as a single i.m. dose. The start of gonadotropin treatment was considered Day 0. Both groups were given 5 mg of Armour Standard LH im on Day 6, and COC were collected by follicle aspiration on Day 7. Expanded COC collected from FSH- (n = 157) and eCG-treated llamas (n = 151) were fertilized in vitro using epididymal sperm, and presumptive zygotes were in vitro cultured in SOF medium for 8 d. The FSH and eCG treatment groups did not differ with respect to: the number of follicles 7 mm (16.0; 2.7 vs 14.0; 1.9, respectively; P = 0.5); the number of COC collected (11.5; 1.9 vs 9.7; 1.2; P = 0.4); the number of expanded COC (9.8; 1.4 vs 9.4; 1.2; P = 0.8); or the percentage of presumptive zygotes which developed into 2 to 8 cell stage embryos (65.3 vs 63.1), morulas (46.2 vs 42.5), or blastocysts (23.1 vs 20.5; P > 0.05). In conclusion, FSH and eCG treatments were equally effective for recovery of a high number of expanded COC which were used directly for in vitro fertilization. Furthermore, rate of embryo development was not significantly affected by the gonadotropin treatment used. © 2011 Elsevier Inc.es
dc.formatPDFes
dc.identifier.citationTheriogenology, Vol. 75, N°8, 1482-1488, 2011es
dc.identifier.doi10.1016/j.theriogenology.2010.11.047es
dc.identifier.urihttps://hdl.handle.net/10925/589
dc.language.isoenes
dc.sourceTheriogenologyes
dc.subjectFertilización in vitroes
dc.subjectSuperestimulaciónes
dc.subjectGonadotropinases
dc.titleIn vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamases
dc.typeArtículo de Revistaes
uct.carreraAgronomíaes
uct.carreraIngeniería en Recursos Naturales Renovableses
uct.carreraMedicina Veterinariaes
uct.catalogadoragaes
uct.comunidadRecursos Naturaleses
uct.facultadFacultad de Recursos Naturaleses
uct.indizacionISIes
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