Protist-Lactic Acid Bacteria Co-Culture as a Strategy to Bioaccumulate Polyunsaturated Fatty Acids in the Protist Aurantiochytrium sp. T66

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datacite.alternateIdentifier.citationMARINE DRUGS,Vol.21,,2023
datacite.alternateIdentifier.doi10.3390/md21030142
datacite.creatorMarileo, Luis
datacite.creatorAcuna, Jacqueline
datacite.creatorRilling, Joaquin
datacite.creatorDiaz, Paola
datacite.creatorLangellotti, Antonio Luca
datacite.creatorRusso, Giovanni Luca
datacite.creatorBarra, Patricio Javier
datacite.creatorDantagnan, Patricio
datacite.creatorViscardi, Sharon
datacite.date2023
datacite.subject.englishAurantiochytrium
datacite.subject.englishPUFA
datacite.subject.englishLAB
datacite.subject.englishco-culture
datacite.subject.englishprotist
datacite.titleProtist-Lactic Acid Bacteria Co-Culture as a Strategy to Bioaccumulate Polyunsaturated Fatty Acids in the Protist Aurantiochytrium sp. T66
dc.date.accessioned2023-06-08T15:48:11Z
dc.date.available2023-06-08T15:48:11Z
dc.description.abstractThraustochytrids are aquatic unicellular protists organisms that represent an important reservoir of a wide range of bioactive compounds, such as essential polyunsaturated fatty acids (PUFAs) such as arachidonic acid (ARA), docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), which are involved in the regulation of the immune system. In this study, we explore the use of co-cultures of Aurantiochytrium sp. and bacteria as a biotechnological tool capable of stimulating PUFA bioaccumulation. In particular, the co-culture of lactic acid bacteria and the protist Aurantiochytrium sp. T66 induce PUFA bioaccumulation, and the lipid profile was evaluated in cultures at different inoculation times, with two different strains of lactic acid bacteria capable of producing the tryptophan dependent auxins, and one strain of Azospirillum sp., as a reference for auxin production. Our results showed that the Lentilactobacillus kefiri K6.10 strain inoculated at 72 h gives the best PUFA content (30.89 mg g(-1) biomass) measured at 144 h of culture, three times higher than the control (8.87 mg g(-1) biomass). Co-culture can lead to the generation of complex biomasses with higher added value for developing aquafeed supplements.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5271
dc.language.isoen
dc.publisherMDPI
dc.sourceMARINE DRUGS
oaire.resourceTypeWOS
oaire.resourceType.enArticle
uct.indizacionSCI
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