Flow cytometry: a tool for understanding the behaviour of polyhydroxyalkanoate accumulators

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datacite.alternateIdentifier.citationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY,Vol.107,581-590,2023
datacite.alternateIdentifier.doi10.1007/s00253-022-12318-x
datacite.creatorGonzalez, Karina
datacite.creatorSalinas, Alejandro
datacite.creatorPinto, Fernanda
datacite.creatorNavia, Rodrigo
datacite.creatorLiu, Shijie
datacite.creatorCea, Mara
datacite.date2023
datacite.subject.englishPolyhydroxyalkanoates
datacite.subject.englishMixed microbial cultures
datacite.subject.englishBioreactors
datacite.subject.englishFlow cytometry
datacite.titleFlow cytometry: a tool for understanding the behaviour of polyhydroxyalkanoate accumulators
dc.date.accessioned2024-05-27T18:26:05Z
dc.date.available2024-05-27T18:26:05Z
dc.description.abstractThe use of mixed microbial cultures (MMCs) is seen as an attractive strategy for polyhydroxyalkanoate (PHA) production. In order to optimize the MMC-PHA production process, tools are required to improve our understanding of the physiological state of the PHA-storing microorganisms within the MMC. In the present study, we explored the use of flow cytometry to analyse the metabolic state and polyhydroxybutyrate (PHB) content of the microorganisms from an MMC-PHA production process. A sequencing batch reactor under a feast and famine regime was used to enrich an MMC with PHB-storing microorganisms. Interestingly, once the PHB-storing microorganisms are selected, the level of PHB accumulation depends largely on the metabolic state of these microorganisms and not exclusively on the consortium composition. These results demonstrate that flow cytometry is a powerful tool to help to understand the PHA storage response of an MMC-PHA production process.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5646
dc.language.isoen
dc.publisherSPRINGER
dc.sourceAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
oaire.resourceTypeArticle
uct.indizacionSCI
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