Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility

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dc.contributor.authorLee Estévez, M.
dc.contributor.authorHerrera, L.
dc.contributor.authorDíaz, R.
dc.contributor.authorBeltrán, J.
dc.contributor.authorFigueroa Villalobos, Elías
dc.contributor.authorDumorné, K.
dc.contributor.authorUlloa Rodríguez, P.
dc.contributor.authorShort, S.
dc.contributor.authorRisopatrón, J.
dc.contributor.authorValdebenito Isler, Iván
dc.contributor.authorFarías, J.
dc.date2019
dc.date.accessioned2020-07-28T22:47:00Z
dc.date.available2020-07-28T22:47:00Z
dc.description.abstractSperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from energetic and cell signalling approaches. In this study, cAMP-dependent protein kinase (PKA) and AMP-activated kinase (AMPK) activities were quantified in fresh and cryopreserved spermatozoa of Atlantic salmon (Salmo salar); and the relation with motility was analysed. Results indicate there was a decrease in membrane integrity and motility in post-thawed spermatozoa compared to fresh samples, however, there was about 30% of cells with intact plasma membrane but incapable of motility. The PKA and AMPK activities were less after cryopreservation, indicating that loss of motility may be related to alteration of these key enzymes. Furthermore, PKA and AMPK activities were positively correlated with each other and with motility; and inhibition decreased motility, indicating there is a functional relationship between PKA and AMPK. The PKA inhibition also decreased AMPK activity, but results from protein-protein docking analyses indicated AMPK activation directly by PKA is unlikely, thus an indirect mechanism may exist. There have been no previous reports of these kinase actions in fish spermatozoa, making these findings worthy of assessment when there are future studies being planned, and may serve as base knowledge for optimization of cryopreservation procedures and development of biotechnologies to improve reproduction efficiency in the aquaculture industryen_US
dc.formatPDFen_US
dc.identifier.citationAnimal Reproduction Science, Vol.209, N° 106133, 2019en_US
dc.identifier.doi10.1016/j.anireprosci.2019.106133en_US
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2277
dc.language.isoenen_US
dc.sourceAnimal Reproduction Scienceen_US
dc.subjectCriopreservaciónen_US
dc.subjectProteína quinasa activadaen_US
dc.subjectMotilidaden_US
dc.subjectEspermatozoidesen_US
dc.subjectSalmo salaren_US
dc.titleEffects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motilityen_US
dc.typeArtículo de Revistaen_US
uct.catalogadorpopen_US
uct.comunidadRecursos Naturalesen_US
uct.indizacionISI - Science Citation Indexen_US
uct.indizacionSCOPUSen_US
uct.nucleosNúcleo en Medio Ambienteen_US
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