Effect of exogenous sperm capacitation inducers on stallion sperm
Date
Authors
SILVA JIMENEZ, MAURICIO
Fuentes, Fernanda B.
Contreras, Maria José
Arroyo-Salvo, Camila A.
Cabrera P, Paulina
Silva, Mauricio Erciario
Merino, Osvaldo
Árias, María Elena
Felmer, Ricardo N.
Fuentes, Fernanda B.
Contreras, Maria José
Arroyo-Salvo, Camila A.
Cabrera P, Paulina
Silva, Mauricio Erciario
Merino, Osvaldo
Árias, María Elena
Felmer, Ricardo N.
Authors
Date
Datos de publicación:
10.1016/j.theriogenology.2024.05.042
Keywords
Capacitation - Equine - Reproduction - Stallion Sperm - Calcium - Calcium - Calcium - Animal - Drug Effect - Horse - Male - Metabolism - Mitochondrial Membrane Potential - Phosphorylation - Physiology - Sperm Capacitation - Spermatozoon - Spermatozoon Motility - Zona Pellucida - Animals - Calcium - Horses - Male - Membrane Potential, Mitochondrial - Phosphorylation - Sperm Capacitation - Sperm Motility - Spermatozoa - Zona Pellucida
Collections
Abstract
Although under appropriate laboratory conditions, sperm from different mammalian species can be capacitated in vitro, the optimal conditions for sperm capacitation in the stallion have been elusive. This study evaluated the effect of different capacitating inducers in Whitten and Tyrode media and assessed their impact on capacitation-related factors. Stallion sperm were incubated with different combinations of capacitating inducers at 38.5 °C in an air atmosphere. Sperm quality variables such as motility, mitochondrial membrane potential, and lipid peroxidation were assessed. Membrane fluidity and intracellular calcium levels were evaluated as early markers of capacitation, while tyrosine phosphorylation events and the sperm's ability to perform acrosomal exocytosis were used as late capacitation markers. Finally, these sperm were evaluated using a heterologous zona pellucida binding assay. The findings confirm that capacitating conditions evaluated increase intracellular calcium levels and membrane fluidity in both media. Similarly, including 2 or 3 inducers in both media increased tyrosine phosphorylation levels and acrosomal exocytosis after exposure to progesterone, confirming that stallion sperm incubated in these conditions shows cellular and molecular changes consistent with sperm capacitation. Furthermore, the zona pellucida binding assay confirmed the binding capacity of sperm incubated in capacitation conditions, a key step for stallion in vitro fertilization success. Further studies are needed to evaluate the effect of these conditions on in vitro fertilization in the horse. © 2024 Elsevier B.V., All rights reserved.
Description
Keywords
Capacitation , Equine , Reproduction , Stallion Sperm , Calcium , Calcium , Calcium , Animal , Drug Effect , Horse , Male , Metabolism , Mitochondrial Membrane Potential , Phosphorylation , Physiology , Sperm Capacitation , Spermatozoon , Spermatozoon Motility , Zona Pellucida , Animals , Calcium , Horses , Male , Membrane Potential, Mitochondrial , Phosphorylation , Sperm Capacitation , Sperm Motility , Spermatozoa , Zona Pellucida
Citation
10.1016/j.theriogenology.2024.05.042