Functional and structural effects of amyloid-beta aggregate on Xenopus laevis oocytes

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datacite.alternateIdentifier.citationMOLECULES AND CELLS,Vol.34,349-355,2012
datacite.alternateIdentifier.doi10.1007/s10059-012-2247-8
datacite.creatorParodi Rivera, Jorge
datacite.creatorOchoa-de la Paz, Lenin
datacite.creatorMiledi, Ricardo
datacite.creatorMartinez-Torres, Ataulfo
datacite.date2012
datacite.subject.englishAlzheimer's disease
datacite.subject.englishATP-currents
datacite.subject.englishtransient-outward chloride current
datacite.subject.englishvoltage-Clamp
datacite.titleFunctional and structural effects of amyloid-beta aggregate on Xenopus laevis oocytes
dc.date.accessioned2021-04-30T16:40:38Z
dc.date.available2021-04-30T16:40:38Z
dc.description.abstractXenopus laevis oocytes exposed to amyloid-beta aggregate generated oscillatory electric activity (blips) that was recorded by two-microelectrode voltage-clamp. The cells exhibited a series of 'spontaneous' blips ranging in amplitude from 3.8 +/- 0.9 nA at the beginning of the recordings to 6.8 +/- 1.7 nA after 15 min of exposure to 1 mu M aggregate. These blips were similar in amplitude to those induced by the channel-forming antimicrobial agents amphotericin B (7.8 +/- 1.2 nA) and gramicidin (6.3 +/- 1.1 nA). The amyloid aggregate-induced currents were abolished when extracellular Ca2+ was removed from the bathing solution, suggesting a central role for this cation in generating the spontaneous electric activity. The amyloid aggregate also affected the Ca2+-dependent Cl- currents of oocytes, as shown by increased amplitude of the transient-outward chloride current (T-out) and the serum-activated, oscillatory Cl- currents. Electron microcopy revealed that amyloid aggregate induced the dissociation of the follicular cells that surround the oocyte, thus leading to a failure in the electro-chemical communication between these cells. This was also evidenced by the suppression of the oscillatory Ca2+-dependent ATP-currents, which require proper coupling between oocytes and the follicular cell layer. These observations, made using the X. laevis oocytes as a versatile experimental model, may help to understand the effects of amyloid aggregate on cellular communication.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3212
dc.language.isoen
dc.publisherKOREAN SOC MOLECULAR & CELLULAR BIOLOGY
dc.sourceMOLECULES AND CELLS
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
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