Characterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)

Simple item page
datacite.alternateIdentifier.citationZygote, Vol.26, N° 6, 443-448, 2018en_US
datacite.alternateIdentifier.doi10.1017/S0967199418000217en_US
datacite.creatorValdebenito Isler, Iván
datacite.creatorFigueroa Villalobos, Elías
datacite.creatorSandoval Vargas, Leydy
datacite.creatorContreras Mellado, Pablo
datacite.creatorSánchez Caamaño, Juan Carlos
datacite.creatorFarías Avendaño, Jorge
datacite.creatorRisopatrón, Jennie
datacite.date2018
datacite.subjectNototheniaen_US
datacite.subjectBlastomerosen_US
datacite.subjectEleginops maclovinusen_US
datacite.subjectRóbalo patagónicoen_US
datacite.titleCharacterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)en_US
dc.date.accessioned2019-12-26T19:32:14Z
dc.date.available2019-12-26T19:32:14Z
dc.description.abstractThere is no information about the characteristics of early cleavage in the Patagonian blennie (Eleginops maclovinus), which can be used as a diagnostic tool for embryo quality. The purpose of this investigation, therefore, was to characterize the first blastomeres of E. maclovinus morphologically. Of a 'pool' of incubated eggs at 10.7 +/- 0.5 degrees C, 100 microphotographs of blastodiscs were extracted at different incubation periods from 0.25 to 5 h after fertilization and analyzed. Blastodiscs taken at 3.5, 4.0 and 5.0 h were characterized and classified into symmetric or asymmetric groups according to their morphology. The proportions of length (L) and width (W) of each blastomere were determined to establish its symmetry. Additionally, 20 microphotographs of blastodiscs of normal appearance were analyzed morphologically (control blastodisc: CB) and compared other blastodiscs (4.0 and 5.0 h). The results showed that before fertilization oocytes presented a somehow turgid aspect (maximum average diameter of 987 +/- 41 mu m) and after fertilization and hydration, their diameter increased to 1001.5 +/- 11 mu m (but not statistically significant) and presented a spherical shape. First cleavage ends after 3.5 h of development, forming two blastomeres 467 +/- 45 mu m length (L) and 328 +/- 21 mu m width (W) with a L/W ratio of 1.43 +/- 0.19. The second cleavage ends after development at 4.5 h forming four blastomeres 238 +/- 65 mu m length and 227 +/- 65 mu m width with a ratio L/W of 1.06 +/- 0.09. Five categories were identified during the blastomere characterization: 70% normal or symmetric; 8% with odd numbers of blastomeres; 6% unequal; 6% 'pie shaped' and 10% amorphousen_US
dc.formatPDFen_US
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/2134
dc.language.isoenen_US
dc.sourceZygoteen_US
oaire.resourceTypeArtículo de Revistaen_US
uct.catalogadorpopen_US
uct.comunidadRecursos Naturalesen_US
uct.indizacionISI - Science Citation Indexen_US
uct.indizacionSCOPUSen_US
uct.nucleosNúcleo en Producción Alimentariaen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Valdebenito_Figueroa_Sandoval_Characterization_2018.pdf
Size:
440.07 KB
Format:
Adobe Portable Document Format
Description:
Lectura de los datos del documento
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
803 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Producción Alimentaria