High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile

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dc.contributor.authorAnabalon, Leonardo
dc.contributor.authorEncina-Montoya, Francisco
dc.contributor.authorSanchez, Pamela
dc.contributor.authorSolano, Jaime
dc.contributor.authorBenavente, Felipe
dc.contributor.authorGuiñez, Basilio
dc.contributor.authorOlivares, Flavio
dc.contributor.authorOberti, Carlos
dc.contributor.authorVega, Rolando
dc.coverageChileen_US
dc.coverageChile
dc.date2019-06
dc.date.accessioned2024-03-14T15:00:08Z
dc.date.available2024-03-14T15:00:08Z
dc.description.abstractEasy, economic, precise species authentication is currently necessary in many areas of research and diagnosis in molecular biology applied to conservation studies of endangered species. Here, we present a new method for the identification of three fox species of the Lycalopex genus in Chile. We developed an assay based on high-resolution melt analysis of the mitochondrial cytochrome B gene, allowing a simple, low cost, fast, and accurate species determination. To validate the assay applicability for noninvasive samples, we collected fecal samples in the Atacama Desert, finding unexpectedly one species outside of its known distribution range. We conclude that the assay has a potential to become a valuable tool for a standardized genetic monitoring of the Lycalopex species in Chile.en_US
dc.description.versionpostprinten_US
dc.formatPDFen_US
dc.identifier.citationECOLOGY AND EVOLUTION,Vol. 9, 7448-7454, 2019en_US
dc.identifier.doi10.1002/ece3.5230en_US
dc.identifier.issn2045-7758en_US
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5529
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rightsObra bajo licencia Creative Commons Atribución 4.0 Internacional en_US
dc.sourceECOLOGY AND EVOLUTIONen_US
dc.subjectBarcodeen_US
dc.subjectLycalopex
dc.subjectHRM
dc.titleHigh-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chileen_US
dc.typeArtículo de Revistaen_US
uct.carreraTecnología Médicaen_US
uct.catalogadorJCCLen_US
uct.comunidadCiencias de la Saluden_US
uct.disciplinaTecnología Médicaen_US
uct.facultadFacultad de Ciencias de la Saluden_US
uct.indizacionSCI EXPANDEDen_US
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