High-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile

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datacite.alternateIdentifier.citationECOLOGY AND EVOLUTION,Vol. 9, 7448-7454, 2019en_US
datacite.alternateIdentifier.doi10.1002/ece3.5230en_US
datacite.alternateIdentifier.issn2045-7758en_US
datacite.creatorAnabalon, Leonardo
datacite.creatorEncina-Montoya, Francisco
datacite.creatorSanchez, Pamela
datacite.creatorSolano, Jaime
datacite.creatorBenavente, Felipe
datacite.creatorGuiñez, Basilio
datacite.creatorOlivares, Flavio
datacite.creatorOberti, Carlos
datacite.creatorVega, Rolando
datacite.date2019-06
datacite.subjectBarcodeen_US
datacite.subjectLycalopex
datacite.subjectHRM
datacite.titleHigh-resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chileen_US
dc.coverageChileen_US
dc.coverageChile
dc.date.accessioned2024-03-14T15:00:08Z
dc.date.available2024-03-14T15:00:08Z
dc.description.abstractEasy, economic, precise species authentication is currently necessary in many areas of research and diagnosis in molecular biology applied to conservation studies of endangered species. Here, we present a new method for the identification of three fox species of the Lycalopex genus in Chile. We developed an assay based on high-resolution melt analysis of the mitochondrial cytochrome B gene, allowing a simple, low cost, fast, and accurate species determination. To validate the assay applicability for noninvasive samples, we collected fecal samples in the Atacama Desert, finding unexpectedly one species outside of its known distribution range. We conclude that the assay has a potential to become a valuable tool for a standardized genetic monitoring of the Lycalopex species in Chile.en_US
dc.formatPDFen_US
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/5529
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rightsObra bajo licencia Creative Commons Atribución 4.0 Internacional en_US
dc.sourceECOLOGY AND EVOLUTIONen_US
oaire.resourceTypeArtículo de Revistaen_US
oaire.versionpostprinten_US
uct.carreraTecnología Médicaen_US
uct.catalogadorJCCLen_US
uct.comunidadCiencias de la Saluden_US
uct.disciplinaTecnología Médicaen_US
uct.facultadFacultad de Ciencias de la Saluden_US
uct.indizacionSCI EXPANDEDen_US
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