Urra, Felipe; Ratto, Marcelo; Silva, Mauricio

Evaluation of the effect of mating, intrauterine deposition of raw seminal plasma or seminal plasma purified beta-NGF on endometrial vascularization in llamas

datacite.alternateIdentifier.citation	THERIOGENOLOGY,Vol.125,18-23,2019
datacite.alternateIdentifier.doi	10.1016/j.theriogenology.2018.10.007
datacite.creator	Urra, Felipe
datacite.creator	Ratto, Marcelo
datacite.creator	Silva, Mauricio
datacite.date	2019
datacite.rights	Acceso Abierto
datacite.subject.english	Llamas
datacite.subject.english	Endometrial vascularization
datacite.subject.english	Semen
datacite.subject.english	Doppler ultrasonography
datacite.title	Evaluation of the effect of mating, intrauterine deposition of raw seminal plasma or seminal plasma purified beta-NGF on endometrial vascularization in llamas
dc.date.accessioned	2021-04-30T16:35:24Z
dc.date.available	2021-04-30T16:35:24Z
dc.description.abstract	The aim of this study was to evaluate the endometrial vascularization area (EVA) of both uterine horns in llamas subjected to different intrauterine treatments resembling physiological conditions after a single mating. Llamas with a growing follicle (>= 8 mm) were randomly assigned to: a) single mating with a fertile male (mating; positive control; n = 6); b) intramuscular administration of 50 mu g of gonadorelin acetate plus an intrauterine infusion of 4 ml of PBS (GnRH; negative control; n = 4); c) intrauterine infusion of 4 ml of raw llama seminal plasma (SP; n = 4) or d) intrauterine infusion of 10 mg of beta-NGF purified from llama semen diluted in 4 ml of PBS (sp beta-NGF: n = 6). Females in GnRH, SP and NGF group received 50% of treatment volume into each horn by guiding an insemination pipet through the cervix. Ovaries were examined by ultrasonography every 12 h until Day2 (Day 0 = Day of treatment) to determine ovulation. Power-Doppler ultrasonography evaluation of EVA in a cross-section of the middle segment of each horn was conducted at 1 h before and 1, 3, 6, 12 and 24 h (intensive evaluation) and 2, 4, 6 and 8 days (long-term evaluation) after treatment administration. Serial EVA data was analyzed as a 2-by-2 factorial design for repeated measures using the MIXED procedure. The analysis included main effects of treatment (mating, SP, sp beta-NGF or GnRH), uterine horn (left vs right), time, and their interactions. According to the 2 by 2 analysis there was no effect of uterine horn on EVA during the first 24 h and from Day 2 to Day 8 after treatment; therefore, data were grouped based on treatment type regardless of uterine horn for both periods of observation. Thus, EVA was affected by time (P < 0.04) and treatment by time interaction (P < 0.02) and tended (P = 0.07) to be influenced by type of treatment during the intensive evaluation period. Females on mating and sp beta-NGF group showed a significant increase in EVA at 3 and 12 h after treatment compared to GnRH and SP groups. However, no effect of treatment, time or their interaction was observed during the long-term evaluation period. In spite of the limited number of animals used in this study, our results allow us to concluded that natural mating and intrauterine deposition of 10 mg of sp beta-NGF induce a symmetrical increase in endometrial vascularization of both uterine horns during the first 24 h post treatment administration in llamas; however, this effect did not persist beyond that period. (C) 2018 Elsevier Inc. All rights reserved.	eng
dc.identifier.doi	10.1016/j.theriogenology.2018.10.007
dc.identifier.issn	0093-691X
dc.identifier.uri	http://repositoriodigital.uct.cl/handle/10925/3195
dc.language.iso	en
dc.publisher	Elsevier
dc.rights	Obra bajo licencia Creative Commons Atribución 4.0 Internacional
dc.source	Theriogenology
oaire.citation.endPage	23
oaire.citation.startPage	18
oaire.citation.volume	125
oaire.citationTitle	Theriogenology
oaire.resourceType	Article
uct.catalogador	bcm
uct.comunidad	Recursos Naturales
uct.facultad	Facultad de Recursos Naturales
uct.indizacion	SCI
uct.indizacion	Scopus