Spermatological characteristics and effects of cryopreservation in Lebranche mullet spermatozoa (Mugil liza Valenciennes, 1836): First report of ultra-rapid freezing

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datacite.alternateIdentifier.citationANIMAL REPRODUCTION SCIENCE,Vol.241,,2022
datacite.alternateIdentifier.doi10.1016/j.anireprosci.2022.106986
datacite.creatorMagnotti, C.
datacite.creatorCerqueira, V.
datacite.creatorVillasante, A.
datacite.creatorRomero, J.
datacite.creatorWatanabe, I.
datacite.creatorOliveira, R. P. S.
datacite.creatorFarias, J.
datacite.creatorMerino, O.
datacite.creatorValdebenito
datacite.creatorFigueroa, E.
datacite.date2022
datacite.subject.englishSpermatology
datacite.subject.englishUltra-rapid freezing
datacite.subject.englishMotility
datacite.subject.englishSperm plasma membrane
datacite.subject.englishMugilidae
datacite.titleSpermatological characteristics and effects of cryopreservation in Lebranche mullet spermatozoa (Mugil liza Valenciennes, 1836): First report of ultra-rapid freezing
dc.date.accessioned2022-07-01T20:39:07Z
dc.date.available2022-07-01T20:39:07Z
dc.description.abstractThe present study investigated the spermatological characteristics of raw semen of Lebranche mullet (Mugil liza), namely pH, and sperm density, and motility; and subsequently evaluated the effects of different times of exposure to cryoprotectants, and the application of an ultra-rapid freezing protocol, on sperm motility and plasma membrane integrity. Semen samples were analyzed undiluted (control) and diluted 1:50 v/v in CF-HBSS + 10% Dimethyl sulfoxide + 30% Ethylene glycol + 94.58 gL-1 Trehalose dehydrate (n = 15). Two treatments - diluted semen samples in cryoprotective medium without ultra-rapid freezing (T1), and diluted semen in cryoprotective medium with ultra-rapid freezing (T2) - were evaluated at 0, 2, 4, 6 and 8 min. The frozen samples were thawed at 37oC for 60 s. The spermatological characteristics recorded for the semen were: pH: 7.57 +/- 0.21; sperm density: 30.4 +/- 2.9 x 109 sperm mL-1; motility: 82 +/- 4.9%. Sperm motility presented differences after 2 min exposure to cryoprotectants (70.0 +/- 2.7%) and ultra-rapid freezing (66.5 +/- 5.8%) compared to the control group (98.5 +/- 1.9% and 98.5 +/- 2.1%, respectively; p < 0.05). On the other hand, the plasma membrane integrity of the spermatozoa after 2 min exposure to cryoprotectants (64.0 +/- 8.6%) and ultra-rapid freezing (62.5 +/- 5.2%) presented no differences compared to the control group (69.5 +/- 3.9% and 70.0 +/- 3.5%, respectively p > 0.05); however, differences were observed in the parameters evaluated after longer exposure and cryopreservation times. This is the first report evaluating the effects of different times of exposure to cryoprotectants and direct ultra-rapid freezing in liquid nitrogen on Mugil liza sperm. Our results demonstrated the protocol of sperm ultra-freezing is safe within a time ' s window of 2 min of exposure to cryoprotectants, after which a toxicity effect on sperm can be observed.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4607
dc.language.isoen
dc.publisherELSEVIER
dc.sourceANIMAL REPRODUCTION SCIENCE
oaire.resourceTypeArticle
uct.indizacionSCI
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