In vitro and in vivo maturation of llama oocytes
| dc.contributor.author | Ratto Fuster, Marcelo | |
| dc.contributor.author | Berland Olea, Marco | |
| dc.contributor.author | Huanca, W. | |
| dc.contributor.author | Singh, J. | |
| dc.contributor.author | Adams, G.P. | |
| dc.date | 2005 | |
| dc.date.accessioned | 2012-03-01T13:54:14Z | |
| dc.date.available | 2012-03-01T13:54:14Z | |
| dc.date.issued | 2012-03-01 | |
| dc.description.abstract | Cumulus-oocyte complexes (COC) were collected from abbatoir-derived llama ovaries and cultured in vitro for 28, 30, or 36 h at 39°C in 5% CO 2 to determine the time required for maturation. The majority of COC (n = 298, 87%) were classified as categories 1 and 2 (COC with ≥5 layers or 2-4 compact layers of cumulus cells, respectively) and homogeneous ooplasm, and the proportion that underwent nuclear maturation (MII) was 78, 81 and 80%, after 28, 30 and 36 h, respectively (P = 0.65). To compare the effectiveness of FSH versus eCG for inducing in vivo maturation, in experiment 2, llamas (n = 20 per group) were treated with: (1) 25 mg FSH, twice-daily for 4 day, plus 5 mg armour of LH at the end of FSH treatment; or (2) 1000 IU of eCG, plus 5 mg armour of LH 4 day after eCG treatment. The FSH- and eCG-treated groups did not differ (P = 0.85) with respect to the number of follicles ≥6 mm at the time of COC collection (17.9 ± 2.2 versus 17.7 ± 2.2), the number of COC collected (10.7 ± 2.1 versus 11.2 ± 2.3 per llama), or the collection rate per follicle aspirated (71 versus 74%). As well, no difference (P = 0.49) was detected between the FSH and eCG groups in the number of expanded COC collected (8.3 ± 2.1 versus 10.6 ± 2.2) or the number of COC at the MII stage (6.9 ± 1.8 versus 8.9 ± 1.9). In conclusion, llama oocytes reached MII as early as 28 h after in vitro culture and both FSH and eCG were equally effective in inducing ovarian superstimulation. Treatment with LH after either FSH or eCG superstimulation permitted the recovery of a preponderance of expanded COC in metaphase II that may be suitable for in vitro fertilization without in vitro maturation. | es |
| dc.format | es | |
| dc.identifier.citation | Theriogenology, Vol.63, N°9, 2445-2457, 2005 | es |
| dc.identifier.doi | 10.1016/j.theriogenology.2004.09.053 | es |
| dc.identifier.uri | https://repositoriodigital.uct.cl/handle/10925/826 | |
| dc.language.iso | en | es |
| dc.source | Theriogenology | es |
| dc.subject | Gonadotropina | es |
| dc.subject | Ovocito | es |
| dc.subject | Camélidos | es |
| dc.title | In vitro and in vivo maturation of llama oocytes | es |
| dc.type | Artículo de Revista | es |
| uct.carrera | Medicina Veterinaria | es |
| uct.catalogador | FGE | es |
| uct.comunidad | Recursos Naturales | es |
| uct.facultad | Facultad de Recursos Naturales | es |
| uct.indizacion | ISI | es |