Effect of cholestanol and cholesterol-loaded cyclodextrin on stallion sperm function and capacitation post-cryopreservation

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datacite.alternateIdentifier.citationTHERIOGENOLOGY,Vol.189,1-10,2022
datacite.alternateIdentifier.doi10.1016/j.theriogenology.2022.06.005
datacite.creatorContreras, Maria Jose
datacite.creatorArias, Maria Elena
datacite.creatorSilva, Mauricio
datacite.creatorCabrera, Paulina
datacite.creatorFelmer, Ricardo
datacite.date2022
datacite.subject.englishStallion sperm
datacite.subject.englishCryopreservation
datacite.subject.englishCapacitation
datacite.subject.englishCyclodextrin
datacite.subject.englishCholestanol
datacite.subject.englishCholesterol
datacite.titleEffect of cholestanol and cholesterol-loaded cyclodextrin on stallion sperm function and capacitation post-cryopreservation
dc.date.accessioned2022-08-17T20:45:21Z
dc.date.available2022-08-17T20:45:21Z
dc.description.abstractCryopreservation of stallion semen is less efficient than other species such as bovine. This is mainly because of the greater susceptibility of stallion sperm to the freezing damage that generates oxidative stress and plasma membrane injury, resulting in DNA fragmentation and cell death.These data suggest the need to develop new strategies of sperm cryopreservation that can improve the efficiency of this technique in stallions by reducing or preventing membrane damage and cell death. The present study aimed to evaluate the effect of adding membrane stabilizers to the freezing medium and assess the quality and in vitro capacitation of stallion sperm after thawing. Semen samples from three stallions frozen with membrane stabilizers (cholesterol-loaded cyclodextrin and cholestanol-loaded cyclodextrin) were evaluated in two experiments: i) sperm quality and functional analysis after thawing, and ii) sperm quality and functional analysis after 4 h of post-thaw incubation in capacitating conditions. Plasma membrane integrity, mitochondrial membrane potential, membrane lipid disorder, intracellular Ca2 thorn , tyrosine phosphorylation, acrosome reaction, DNA damage, sperm motility, and binding to the zona pellucida were assessed. The results showed that cholesterol-loaded cyclodextrin was the stabilizer that most efficiently reduced the membrane disruption and post-thaw cell damage. In addition, this stabilizer made it possible to obtain in vitro capacitated sperm showing higher plasma membrane integrity, mitochondrial membrane potential, sperm motility, binding to the zona pellucida and better response to in vitro capacitating conditions. (c) 2022 Elsevier Inc. All rights reserved.
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/4660
dc.language.isoen
dc.publisherELSEVIER SCIENCE INC
dc.sourceTHERIOGENOLOGY
oaire.resourceTypeArticle
uct.indizacionSCI
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