Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function

Simple item page
datacite.alternateIdentifier.citationFISH PHYSIOLOGY AND BIOCHEMISTRY,Vol.39,1215-1221,2013
datacite.alternateIdentifier.doi10.1007/s10695-013-9777-7
datacite.creatorParedes, Marco
datacite.creatorGonzález Alfaro, Katerina
datacite.creatorFigueroa, Jaime
datacite.creatorMontiel-Eulefi, Enrique
datacite.date2013
datacite.subject.englishSalmo salar
datacite.subject.englishProlactin
datacite.subject.englishPhagocytic activity
datacite.subject.englishSuperoxide anion
datacite.titleImmunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function
dc.date.accessioned2021-04-30T16:43:34Z
dc.date.available2021-04-30T16:43:34Z
dc.description.abstractThe in vitro and in vivo effect of prolactin (PRL) on kidney macrophages from Atlantic salmon (Salmo salar) was investigated under the assumption that PRL stimulates immune innate response in mammals. Kidney macrophages were treated two ways: first, cultured in RPMI 1640 medium containing 10, 25, 50 and 100 ng/mL of PRL and second, isolated from a fish with a PRL-injected dose of 100 ng/Kg. Reduced nitro blue tetrazolium (formazan) was used to produce intracellular superoxide anion. Phagocytic activity of PRL was determined in treated cells by optical microscopy observation of phagocytized Congo red-stained yeast. Kidney lysozyme activity was measured in PRL-injected fish. In vitro and in vivo macrophages treated with PRL presented an enhanced superoxide anion production, elevated phagocytic index and increased phagocytic activity. Treated fish showed higher levels of lysozyme activity in the head kidney compared to the control. These results indicate that PRL-stimulated innate immune response in Atlantic salmon and future studies will allow us to assess the possibility of using PRL as an immunostimulant in the Chilean salmon industry.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/3346
dc.language.isoen
dc.publisherSPRINGER
dc.sourceFISH PHYSIOLOGY AND BIOCHEMISTRY
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
Files
Collections
Migración Web of Science