Serotypes of listeria monocytogenes isolated on food produced in cautin province, Chile

dc.contributor.authorBetancourt, O.
dc.contributor.authorVillagrán, K.
dc.contributor.authorMuñoz, F.
dc.contributor.authorGutierrez, E.
dc.contributor.authorMayorga, M.
dc.contributor.authorMelgarejo, P.
dc.date2010
dc.date.accessioned2012-03-04T04:50:41Z
dc.date.available2012-03-04T04:50:41Z
dc.date.issued2012-03-04
dc.description.abstractIt has been reported in Chile that L. monocytogenes is present in differents kind of foods. The aim of this study was to obtain information of the occurrence of the bacteria in sausages, raw milk and vegetables produced in Cautín Province, and to know the L. monocytogenes serotypes involved. For this purpose sausages, raw milk and several vegetables samples, were collected on 2003. For the isolation and identification of L. monocytogenes, the samples were grown in tripticase soy broth (TSB) inoculated into a preenrichment broth for Listeria (35°C followed by an inoculation on selectives medias Oxford and Palcam (35-37°C). Biochemical test were performed to all the typical Listeria colonies according to the methodology recommended by Bacteriological Analytical Manual (FDA). Serotyping was carried out using commercial specific antisera on 53 bacterial strains. Results of the analysis indicated the presence of L. monocytogenes in 61.1% of sausages samples, 0.0% in raw milk samples and in 16.6% of vegetables samples. These results could indicate failure in the hygiene practices, like cleaning and sanitizing the surface in contact with the food. The presence of serotipe 4e in 3 strains were found in some sausages. There was a strain of virulent serotype 1/2a, 1/2b and 4b, the first and second resulted isolated from sausages and the third one resulted from vegetables. The presence of serotypes 1/2a, 1/2b and 4b, is a threat of an eventual out-break, especially in persons quite susceptive, that is why, there is a huge risk for pubic health in customers from this region. These results represent for chilean publićs health authorities an enormous challenge for controlling and prevention procedures.es
dc.formatPDFes
dc.identifier.citationRevista Cientifica de la Facultad de Ciencias Veterinarias de la Universidad del Zulia , Vol. 20, Nº5, 529-536, 2010es
dc.identifier.urihttps://repositoriodigital.uct.cl/handle/10925/845
dc.language.isoenes
dc.sourceRevista Cientifica de la Facultad de Ciencias Veterinarias de la Universidad del Zuliaes
dc.subjectL. monocytogeneses
dc.subjectLonganizases
dc.subjectLeche crudaes
dc.subjectHortalizases
dc.titleSerotypes of listeria monocytogenes isolated on food produced in cautin province, Chilees
dc.title.alternativeSerotipos de listeria monocytogenes aislados de alimentos producidos en la Provincia de Cautín, Chilees
dc.typeArtículo de Revistaes
uct.carreraAgronomíaes
uct.catalogadorpopes
uct.comunidadRecursos Naturaleses
uct.facultadFacultad de Recursos Naturaleses
uct.indizacionISIes
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