Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))
Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))
Authors
Dumorne, Kelly
Valdebenito, Iván
Contreras, Pablo
Ulloa Rodríguez, Patricio
Risopatrón, Jennie
Figueroa, Elías
Estévez, Manuel Lee
Díaz, Rommy
Farías, Jorge
Valdebenito, Iván
Contreras, Pablo
Ulloa Rodríguez, Patricio
Risopatrón, Jennie
Figueroa, Elías
Estévez, Manuel Lee
Díaz, Rommy
Farías, Jorge
Authors
Date
2019-11-05
Datos de publicación:
10.1016/j.aqrep.2018.05.002
Keywords
Genypterus blacodes - Fertilidad - Espermatozoides
Collections
Abstract
In this research we evaluated simple aspects of the sperm biology of Genypterus blacodes, in particular assessing the effects of pH (6, 7, 8 and 9), temperature (4, 8 and 16 degrees C) and osmolalities 100% sea water (1010 mOsm/kg, Control), 75% sea water (774 mOsm/kg, T-1), 50% sea water (488 mOsm/kg, T-2) and distilled water (0 mOsm/kg, T-3)) on the motility of Genypterus blacodes intratesticular spermatozoa.
In addition, we determined the fertilization rate. Our results show that G. blacodes spermatozoa have a sperm density of 5.35 +/- 0.16 x 10(9) spermatozoa/mL. Sperm motility is initiated on contact with a hyperosmotic swimming medium under normal conditions (1010 mOsm/kg, pH 8 and 8 degrees C). The longest motility duration (432.48 +/- 8.89 s) was recorded at 4 degrees C. The maximum percentage of motile cells was recorded at 8 degrees C (65.66 +/- 4.95) at osmolality 1010 mOsm/kg, whereas an optimum was observed at pH 8. The fertility rate was 73.9 +/- 17%. This is the first report on sperm motility of G. blacodes spermatozoa. In conclusion, the results of this study permit a baseline to be established for further research and protocols for artificial reproduction of this species to be developed and optimized. In addition, the information gathered in this research will be useful for developing the biotechnology of Genypterus blacodes