Cell Junction Pathology of Neural Stem Cells Is Associated With Ventricular Zone Disruption, Hydrocephalus, and Abnormal Neurogenesis

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datacite.alternateIdentifier.citationJOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY,Vol.74,653-671,2015
datacite.alternateIdentifier.doi10.1097/NEN.0000000000000203
datacite.creatorMontserrat Guerra, Maria
datacite.creatorHenzi, Roberto
datacite.creatorOrtloff Trautmann, Alexander
datacite.creatorLichtin, Nicole
datacite.creatorVio, Karin
datacite.creatorJimenez, Antonio J.
datacite.creatorDolores Dominguez Pinos, Maria
datacite.creatorGonzalez, Cesar
datacite.creatorClara Jara, Maria
datacite.creatorHinostroza, Fernando
datacite.creatorRodriguez, Sara
datacite.creatorJara, Maryoris
datacite.creatorOrtega, Eduardo
datacite.creatorGuerra, Francisco
datacite.creatorSival, Deborah A.
datacite.creatorden Dunnen, Wilfred F. A.
datacite.creatorPerez Figares, Jose M.
datacite.creatorMcAllister, James P.
datacite.creatorJohanson, Conrad E.
datacite.creatorRodriguez, Esteban M.
datacite.date2015
datacite.subject.englishCerebrospinal fluid
datacite.subject.englishCongenital hydrocephalus
datacite.subject.englishHTx rat
datacite.subject.englishHuman
datacite.subject.englishJunction pathology
datacite.subject.englishNeural stem cells
datacite.subject.englishNeurospheres
datacite.subject.englishVentricular zone disruption
datacite.titleCell Junction Pathology of Neural Stem Cells Is Associated With Ventricular Zone Disruption, Hydrocephalus, and Abnormal Neurogenesis
dc.date.accessioned2021-04-30T17:06:06Z
dc.date.available2021-04-30T17:06:06Z
dc.description.abstractFetal-onset hydrocephalus affects 1 to 3 per 1,000 live births. It is not only a disorder of cerebrospinal fluid dynamics but also a brain disorder that corrective surgery does not ameliorate. We hypothesized that cell junction abnormalities of neural stem cells (NSCs) lead to the inseparable phenomena of fetal-onset hydrocephalus and abnormal neurogenesis. We used bromodeoxyuridine labeling, immunocytochemistry, electron microscopy, and cell culture to study the telencephalon of hydrocephalic HTx rats and correlated our findings with those in human hydrocephalic and nonhydrocephalic human fetal brains (n = 12 each). Our results suggest that abnormal expression of the intercellular junction proteins N-cadherin and connexin-43 in NSC leads to 1) disruption of the ventricular and subventricular zones, loss of NSCs and neural progenitor cells; and 2) abnormalities in neurogenesis such as periventricular heterotopias and abnormal neuroblast migration. In HTx rats, the disrupted NSC and progenitor cells are shed into the cerebrospinal fluid and can be grown into neurospheres that display intercellular junction abnormalities similar to those of NSC of the disrupted ventricular zone; nevertheless, they maintain their potential for differentiating into neurons and glia. These NSCs can be used to investigate cellular and molecular mechanisms underlying this condition, thereby opening the avenue for stem cell therapy.
dc.identifier.urihttp://repositoriodigital.uct.cl/handle/10925/4028
dc.language.isoen
dc.publisherOXFORD UNIV PRESS INC
dc.sourceJOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
oaire.resourceTypeArticle
uct.catalogadorWOS
uct.indizacionSCI
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