Short-term storage of salmonids semen in a sodium alginate-based extender
Date
Authors
FIGUEROA VILLALOBOS, ELIAS GUSTAVO
VALDEBENITO ISLER, NEMESIO IVAN
Merino, Osvaldo
Villalobos, Elías Figueroa
Cheuquemán, C.
Valdebenito, Iván
Isachenko, Vladimir V.
Isachenko, Evgenia
Sánchez G., Raúl Segundo
Farias, Jorge G.
Risopatrón, Jennie
VALDEBENITO ISLER, NEMESIO IVAN
Merino, Osvaldo
Villalobos, Elías Figueroa
Cheuquemán, C.
Valdebenito, Iván
Isachenko, Vladimir V.
Isachenko, Evgenia
Sánchez G., Raúl Segundo
Farias, Jorge G.
Risopatrón, Jennie
Authors
Date
Datos de publicación:
10.1111/and.12661
Keywords
Antioxidants - Fish - Semen Storage - Sodium Alginate - Sperm Function - Alginic Acid - Superoxide - Glucuronic Acid - Alginates - Alginic Acid - Glucuronic Acid - Hexuronic Acids - Superoxides - Storfish - Storfish Ext-c - Alginic Acid - Free Radical - Preservative - Superoxide - Glucuronic Acid - Hexuronic Acid - Animal Experiment - Article - Cell Membrane - Cell Viability - Controlled Study - Dna Fragmentation - Male - Mitochondrial Membrane Potential - Nonhuman - Oncorhynchus Kisutch - Oncorhynchus Mykiss - Oxidative Stress - Salmo Salar - Salmonid - Sperm - Sperm Preservation - Sperm Quality - Spermatozoon - Spermatozoon Motility - Animal - Cell Survival - Procedures - Time Factor - Veterinary - Alginates - Animals - Cell Survival - Dna Fragmentation - Glucuronic Acid - Hexuronic Acids - Male - Membrane Potential, Mitochondrial - Salmonidae - Semen Preservation - Sperm Motility - Superoxides - Time Factors
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Abstract
Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our study was to analyse the effect of a sodium alginate-based extender on sperm function in the short-term storage of salmonids semen. Samples of Salmo salar, Oncorhynchus kisutch, and Oncorhynchus mykiss were stored in Storfish® (Ext-C) and Storfish® supplemented with sodium alginate (Ext-A) during 10 days at 4°C. After storage, motility, viability, mitochondrial membrane potential (??mit), superoxide anion (O<inf>2</inf> ?) level and DNA fragmentation (DNA Frag) were assessed. Ext-A had positive effect in preservation of sperm motility, viability, ??mit, O<inf>2</inf> ? level and DNA integrity in the three species analysed compared to control samples. In Ext-A, the spermatozoa of S. salar and O. mykiss showed significantly higher motility, viability and ??mit than O. kisutch. However, O. kisutch and O. mykiss had significantly lower O<inf>2</inf> ? level than S. salar, and DNA fragmentation in O. kisutch and S. salar was significantly lower than in samples of O. mykiss (p < 0.05). Dilution of salmonids semen in a sodium alginate-based extender is effective for protecting sperm quality during 10 days of short-term storage. © 2017 Elsevier B.V., All rights reserved.
Description
Keywords
Antioxidants , Fish , Semen Storage , Sodium Alginate , Sperm Function , Alginic Acid , Superoxide , Glucuronic Acid , Alginates , Alginic Acid , Glucuronic Acid , Hexuronic Acids , Superoxides , Storfish , Storfish Ext-c , Alginic Acid , Free Radical , Preservative , Superoxide , Glucuronic Acid , Hexuronic Acid , Animal Experiment , Article , Cell Membrane , Cell Viability , Controlled Study , Dna Fragmentation , Male , Mitochondrial Membrane Potential , Nonhuman , Oncorhynchus Kisutch , Oncorhynchus Mykiss , Oxidative Stress , Salmo Salar , Salmonid , Sperm , Sperm Preservation , Sperm Quality , Spermatozoon , Spermatozoon Motility , Animal , Cell Survival , Procedures , Time Factor , Veterinary , Alginates , Animals , Cell Survival , Dna Fragmentation , Glucuronic Acid , Hexuronic Acids , Male , Membrane Potential, Mitochondrial , Salmonidae , Semen Preservation , Sperm Motility , Superoxides , Time Factors
Citation
10.1111/and.12661