Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species
Date
Authors
FIGUEROA VILLALOBOS, ELIAS GUSTAVO
VALDEBENITO ISLER, NEMESIO IVAN
Magnotti, Caio
Cerqueira, Vinicius
Lee-Estevez, Manuel
Farias, Jorge G.
Valdebenito, Ivan
Figueroa, Elias
VALDEBENITO ISLER, NEMESIO IVAN
Magnotti, Caio
Cerqueira, Vinicius
Lee-Estevez, Manuel
Farias, Jorge G.
Valdebenito, Ivan
Figueroa, Elias
Authors
Date
Datos de publicación:
10.1111/raq.12145
Keywords
cryoprotectant - extender - milt - slow freezing - spermatozoa
Collections
Abstract
Semen conservation methods have been extensively studied for fish species over the last 60years. Cryopreservation techniques can be divided into two types: one with slow cooling rates like traditional freezing and the other with ultrarapid rates as used in vitrification. These protocols increase the time over which semen samples can be used for reproduction or sperm quality analysis. Marine fish possess greater resistance than freshwater species to variations in osmolality. This favours the application of these methods, which produce better results after thawing and offer high biotechnological potential for aquaculture. In the last 15years, sperm cryopreservation studies have been carried out in 32 marine species, but there are few analyses of the effects of freezing on the morphology and physiology of sperm cells. The object of this review was to provide recent data on semen cryopreservation in marine fish species and to suggest variables which may be applied in future research.
Description
Keywords
cryoprotectant , extender , milt , slow freezing , spermatozoa
Citation
10.1111/raq.12145